Effects of curcumin on proliferation, cell cycle and apoptosis of human thyroid cancer TPC-1 cells
10.3760/cma.j.cn231583-20230322-00064
- VernacularTitle:姜黄素对人甲状腺癌TPC-1细胞增殖、周期和凋亡的影响
- Author:
Yuli WANG
1
;
Zhitao GUO
;
Yaguang FAN
Author Information
1. 天津医科大学总医院医学检验科，天津 300052
- Keywords:
Curcumin;
Thyroid cancer;
Proliferation;
Cell cycle;
Apoptosis
- From:
Chinese Journal of Endemiology
2023;42(12):984-988
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of curcumin on proliferation, cell cycle, and apoptosis of human thyroid cancer TPC-1 cells.Methods:Logarithmic growth phase TPC-1 cells were treated with 0.0, 7.5, 15.0 and 22.5 μmol/L curcumin for 48 h. Cell proliferation levels were determined using the methylthiazolyl tetrazolium (MTT) assay. Cell cycle and apoptosis levels were measured using flow cytometry. Western blot method was used to determine the expression levels of B cell lymphoma-2 (Bcl-2) and Bcl-2-associated X protein (Bax).Results:The inhibition rates of cell proliferation in 7.5, 15.0 and 22.5 μmol/L curcumin groups were higher than that of 0.0 μmol/L curcumin group ( P < 0.05); the proportion of G0/G1 phase was higher than that of 0.0 μmol/L curcumin group, but the proportions of G2/M and S phase were lower than those of 0.0 μmol/L curcumin group ( P < 0.05); the cell apoptosis rate was higher than that of 0.0 μmol/L curcumin group [(14.13 ± 0.57)%, (25.27 ± 0.62)%, (36.01 ± 0.84)% vs (8.48 ± 0.43)%, P < 0.05]; the protein expression of Bcl-2 was lower than that of 0.0 μmol/L curcumin group, and protein expression of Bax was higher than that of 0.0 μmol/L curcumin group ( P < 0.05). Conclusion:Curcumin can inhibit the proliferation of TPC-1 cells, block the cell cycle in G0/G1 phase, and promote cell apoptosis by downregulating Bcl-2 expression and upregulating Bax expression.
