Rapid culture and identification of mouse primary thyroid cells
10.3969/j.issn.1000-4718.2024.03.023
- VernacularTitle:小鼠甲状腺原代细胞的快速培养和鉴定
- Author:
Qiuchan TAN
1
;
Jiawei LIN
;
Xiaoya YANG
;
Li PAN
;
Dandan YAO
;
Liwei WANG
;
Lixin CHEN
;
Zhuhua WU
Author Information
1. 广州卫生职业技术学院生理学教研室,广东 广州 510450
- Keywords:
thyroid;
endocrine;
primary culture;
mouse
- From:
Chinese Journal of Pathophysiology
2024;40(3):572-576
- CountryChina
- Language:Chinese
-
Abstract:
AIM:The paper is to explore a rapid and simple method for the culture of mouse primary thyroid cells.METHODS:Mouse thyroid cells were isolated by enzyme digestion and cultured with improved medium,and their morphology,characteristics and secretory function were observed within 14 d.RESULTS:In the cultures,the active pri-mary cells were obtained from the thyroid tissue after digestion for 25 min;adherent growth was observed on the 2nd day.And secondary follicles appeared from the 5th to 7th day.Over 95%cells were detected with thyroglobulin.The secretion of total triiodothyronine and total thyroxine maintains over 60%in 7 d.The expression levels of specific genes can still maintain more than 50%in 10 d.CONCLUSION:Mouse thyroid primary cells can be rapidly cultured by this method,and the cells can be used for studying thyroid endocrine secretion within 7 d and studying thyroid genes within 10 d.
