Mdivi-1 protects oligodendrocytes through inhibiting apoptotic signaling pathway
10.3969/j.issn.1000-4718.2024.00.011
- VernacularTitle:Mdivi-1通过抑制少突胶质细胞凋亡信号通路发挥髓鞘保护作用
- Author:
Yanhua LI
1
;
Xiaojuan ZHANG
;
Siyu ZHANG
;
Xiyuan HOU
;
Ziyi LIU
;
Xiao-Jing YU
;
Nianping ZHANG
Author Information
1. 山西大同大学医学院,老年慢性病智慧医康养大同市重点实验室,山西 大同 037009
- Keywords:
mitochondrial fission inhibitor-1;
multiple sclerosis;
experimental autoimmune encephalomyeli-tis;
apoptosis;
oligodendrocytes
- From:
Chinese Journal of Pathophysiology
2024;40(3):527-534
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the therapeutic effect of mitochondrial fission inhibitor-1(Mdivi-1)on experi-mental autoimmune encephalomyelitis(EAE)in mice,and to explore its mechanism.METHODS:The mice immunized with myelin oligodendrocyte glycoprotein peptide fragment 35-55(MOG35-55)were randomly divided into DMSO model group and Mdivi-1 intervention group.All mice were sacrificed on the 28th day after the first immunization.The demyelination was analyzed by Luxol fast blue staining.The protective mechanism of Mdivi-1 in the spinal cord tissue was investigated by immunofluorescence staining,TUNEL staining and the in vitro experiment with MO3.13 oligodendrocytes treated with staurosporine.The mitochondrial depolarization was detected by JC-1 staining,the cell injury was checked by LDH leakage,and the viability of MO3.13 oligodendrocytes was determined by MTT assay.RESULTS:Compared with DMSO model group,the demyelinating injury was alleviated and the proportion of apoptotic CC1+ oligodendrocytes in Mdivi-1 group was decreased.The cleaved caspase-3,caspase-9,cytochrome C and Bax protein expression levels in the spinal cord of Mdivi-1-treated mice was also attenuated.The in vitro MO3.13 cell experiments suggested that Mdivi-1 inhibited MO3.13 cell mitochondrial depolarization,attenuated the cell damage and increased the cell viability.CONCLUSION:Mdivi-1 pro-tects against the myelin injury in EAE mice,which may be related to the suppression of oligodendrocyte apoptosis.
