ATP5J regulates mitochondrial function through TOMM20 and promotes metastasis of human hepatocellular carcinoma cells
10.3969/j.issn.1000-4718.2024.03.006
- VernacularTitle:ATP5J通过TOMM20调节线粒体功能并促进人肝细胞癌细胞转移
- Author:
Junzhi LENG
1
;
Genwang WANG
;
Di LIU
;
Kejun LIU
;
Qi WANG
;
Yongfeng HUI
Author Information
1. 宁夏医科大学总医院肝胆外科,宁夏 银川 750004
- Keywords:
hepatocellular carcinoma;
mitochondrial function;
ATP5J protein;
cytoskeleton
- From:
Chinese Journal of Pathophysiology
2024;40(3):431-437
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the mechanism of ATP synthase mitochondrial F0 complex H+ transporting,sub-unit F6(ATP5J)in affecting the metastasis of hepatoma carcinoma cells by regulating mitochondrial function-mediated cy-toskeletal remodeling.METHODS:Hepatocellular carcinoma cells Li-7 were used to construct the ATP5J overexpression and knockdown models.JC-1 staining was used to detect the mitochondrial membrane potential in each group,reactive oxygen species(ROS)levels were examined by DCHF-DA,and mitochondrial ATP fluorescence probe was used to assess mito-chondrial function.Cytoskeletal remodeling was detected with a microfilament green fluorescent probe(Actin-Tracker Green-488).Transwell assay was used to assess cell invasion ability.The expression levels of ATP5J and translocase of outer mitochondrial membrane 20(TOMM20)were determined by Western blot.RESULTS:Overexpression of ATP5J up-regulated mitochondrial membrane potential and mitochondrial ATP fluorescence intensity,induced cytoskeletal re-modeling,promoted cell invasion and TOMM20 expression,and inhibited ROS production(P＜0.01).On the contrary,knockdown of ATP5J significantly decreased mitochondrial membrane potential and mitochondrial ATP fluorescence inten-sity,significantly decreased cell invasion ability and TOMM20 expression,promoted ROS production and blocked cyto-skeletal remodeling(P＜0.01).CONCLUSION:ATP5J regulates mitochondrial energy transformation in hepatocellular carcinoma cells,and affects metastasis of hepatoma carcinoma cells by regulating mitochondrial membrane potential and mitochondrial ATP production-mediated cytoskeletal remodeling through TOMM20.
