Total flavonoids of Pterocarya hupehensis Skan inhibit migration and in-vasion of fibroblast-like synoviocytes by regulating PI3K/AKT signaling pathway
10.3969/j.issn.1000-4718.2024.00.007
- VernacularTitle:湖北枫杨总黄酮通过调控PI3K/AKT信号通路抑制成纤维细胞样滑膜细胞的迁移和侵袭
- Author:
Hao WU
1
;
Guoqing CHEN
;
Man LU
;
Ying GAO
;
Qiling JIANG
;
Haonan LUO
;
Ling YUAN
;
Yang XIANG
Author Information
1. 湖北恩施学院医学部,湖北民族大学风湿性疾病发生与干预湖北省重点实验室,湖北 恩施 445000
- Keywords:
total flavonoids of Pterocarya hupehensis Skan;
fibroblast-like synoviocytes;
rheumatoid arthri-tis;
PI3K/AKT signaling pathway
- From:
Chinese Journal of Pathophysiology
2024;40(1):134-140
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe how total flavonoids of Pterocarya hupehensis Skan(PHSTF)affects the migra-tion and invasion of human rheumatoid fibroblast-like synoviocyte line MH7A.METHODS:The MH7A cells were divided into control group(without any treatment),low-,medium-and high-dose(6.25,12.5 and 25 mg/L,respectively)PHSTF groups,phosphatidylinositol 3-kinase(PI3K)inhibitor 740Y-P(10 μmol/L)group,and 740Y-P(10 μmol/L)+high-dose(25 mg/L)PHSTF group.The viability of the MH7A cells was determined by CCK-8 assay,while the migration and inva-sion were assessed by scratch and Transwell assays.The protein levels of matrix metalloproteinase-2(MMP-2),MMP-9,PI3K,p-PI3K,AKT and p-AKT were detected by Western blot.RESULTS:Compared with control group,the cell via-bility in PHSTF treatment groups was reduced(P<0.05),the cell wound healing area was significantly decreased(P<0.01),migratory and invasive cells in the lower chamber were significantly reduced(P<0.01),and the protein expres-sion of MMP-2 and MMP-9 and the ratios of p-PI3K/PI3K and pAKT/AKT were decreased(P<0.01).Compared with high-dose PHSTF group,the addition of PI3K/AKT pathway agonist 740Y-P significantly increased the migration and invasion ability of MH7A cells(P<0.01)and elevated the protein expression of MMP-2 and MMP-9 and the ratios of p-PI3K/PI3K and pAKT/AKT(P<0.01)under the treatment with PHSTF.CONCLUSION:Total flavonoids of Pterocarya hupehensis Skan could inhibit the migration and invasion of MH7A cells by regulating the PI3K/AKT signaling pathway.
