Effect of Xiaoxuming decoction on OGD/R-induced synaptic plasticity in HT22 cells
10.3969/j.issn.1000-4718.2024.01.016
- VernacularTitle:小续命汤对OGD/R诱导HT22细胞损伤后突触可塑性的影响
- Author:
Manman WANG
1
,
2
;
Rui LAN
;
Yong ZHANG
;
Xueqin FU
;
Xuhuan ZOU
;
Weiwei WANG
;
Hongyu LI
;
Chen TANG
;
Shuang LIU
Author Information
1. 河南中医药大学第一临床医学院, 河南 郑州 450046
2. 河南中医药大学第一附属医院脑病中心,河南 郑州 450099
- Keywords:
Xiaoxuming decoction;
traditional medicinal formula;
OGD/R;
HT22;
ischemic stroke;
synap-tic plasticity
- From:
Chinese Journal of Pathophysiology
2024;40(1):126-133
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the protective effect of Xiaoxuming decoction(XXMD)on synaptic plasticity in the context of cerebral ischemia-reperfusion injury following ischemic stroke.METHODS:An oxygen-glucose depriva-tion/reoxygenation(OGD/R)model was employed in vitro using mouse hippocampal neurons(HT22 cells)to simulate ischemia-reperfusion injury.Cell viability was assessed using a CCK-8 assay to determine the optimal XXMD concentra-tion.The HT22 cells were divided into two groups:control and model(OGD/R).Cellular morphological changes were ob-served using an inverted microscope.The levels of IL-1β,IL-6 and TNF-α in the supernatant were quantified by ELISA.Ultrastructural changes were examined by transmission electron microscopy.Immunofluorescence staining was used to de-tect neuron markers NeuN and synaptic proteins NF200 and MAP2.The protein levels of NF200 and MAP2 were analyzed by Western blot.RESULTS:The highest cell survival rate occurred at an XXMD concentration of 100 mg/L(P<0.05).Compared with control group,the cells in model group exhibited round shape and shrinkage,mitochondrial swelling or vacuolization,and a marked decrease in survival rate.There were significant increases in IL-1β,IL-6 and TNF-α levels(P<0.05).Immunofluorescence intensity and protein levels of NeuN,NF200 and MAP2 were notably reduced(P<0.05).Treatment with XXMD improved cell morphology,ultrastructure and survival rate(P<0.05),and decreased in-flammatory factor levels(P<0.05).Compared with model group,the cells in OGD/R+XXMD group showed significantly increased immunofluorescence intensity and protein levels of NeuN,NF200 and MAP2(P<0.05).CONCLUSION:Xiaoxuming decoction may mitigate OGD/R-induced injury,potentially by inhibiting inflammatory responses and enhanc-ing synaptic plasticity.
