Effects of Prunus cerasifera Ehrh.polyphenol extract on glucose metabo-lism in insulin-resistant HepG2 cells based on AMPK signaling pathway
10.3969/j.issn.1000-4718.2023.12.014
- VernacularTitle:基于AMPK信号通路探究樱桃李多酚提取物对胰岛素抵抗HepG2细胞糖代谢的作用
- Author:
PAERHATI SUHEIYAN
1
;
Jiaojiao ZHANG
;
Junren WANG
;
Jiaqi ZHAO
;
Yanhong LI
Author Information
1. 新疆特殊环境物种多样性应用与调控实验室,新疆特殊环境物种保护与调控生物学实验室,新疆师范大学生命科学学院,新疆 乌鲁木齐 830054
- Keywords:
Prunus cerasifera Ehrh.polyphenol extracts;
HepG2 cells;
insulin resistance;
glucose metabo-lism;
AMP-activated protein kinase
- From:
Chinese Journal of Pathophysiology
2023;39(12):2234-2241
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of Prunus cerasifera Ehrh.polyphenol extract(PCE)on glucose metabolism in insulin-resistant HepG2(IR-HepG2)cells.METHODS:An IR-HepG2 cells model was established using high sugar and high insulin induction;a control group,an insulin resistance(IR)model group,a PCE treatment group with different concentrations,and a metformin treatment group were set up.Cells were treated with 50,100,200,and 400 mg/L of polyphenol extract for 24 hours,respectively,to detect their effects on cellular glucose consumption.At the same time,use thiazole blue(MTT)colorimetry to evaluate cell viability.Real-time PCR and Western blot were used to detect expression of glucose metabolism-related mRNA and proteins.RESULTS:Prunus cerasifera Ehrh.polyphenol ex-tract can significantly increase glucose consumption in IR-HepG2 cells,and cell activity tends to increase and then decline as its concentration increases;At mRNA and protein levels,in addition to significantly increasing the expression of phos-phorylated adenosine monophosphate activated protein kinase(p-AMPK)protein in IR-HepG2 cells,it also decrease gly-cogen synthase kinase 3β(GSK3β)in glycogen synthesis and gluconeogenesis pathways,forked protein O1(FoxO1),peroxisome proliferator activates gamma co-receptor activation 1-alpha(PGC-1α),glucose-6-phosphatase(G6Pase)and phosphoenol pyruvate carboxykinase(PEPCK)expression.CONCLUSION:Prunus cerasifera Ehrh.polyphenol extract can activate the AMPK signaling pathway in IR-HepG2 cells,to further inhibit gluconeogenesis and glycogen synthesis pathways,thereby improving the insulin resistance state of HepG2 cells.
