DLL3 promotes progression of prostate cancer through MAPK pathway
10.3969/j.issn.1000-4718.2023.00.002
- VernacularTitle:DLL3通过调节MAPK通路促进前列腺癌进展
- Author:
Yuxuan LI
1
,
2
;
Lina YU
;
Weiyang ZHANG
;
Jingru QI
;
Zhuoyang ZHAO
;
Xing HUA
Author Information
1. 暨南大学基础医学与公共卫生学院病理学系,广东 广州 510632
2. 暨南大学附属广州市红十字会医院病理科,广东 广州 510220
- Keywords:
prostate cancer;
Delta-like ligand 3;
epithelial-mesenchymal transition;
MAPK pathway
- From:
Chinese Journal of Pathophysiology
2023;39(12):2165-2175
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the impact of Delta-like ligand 3(DLL3)on the progression of prostate cancer(Pca)and to elucidate its potential molecular mechanisms.METHODS:Overexpression and interference plasmids of DLL3 gene were constructed,and the effects of DLL3 on the proliferation,migration and invasion of Pca cells were as-sessed.Tumorigenesis assay was employed to determine whether DLL3 influenced the proliferation of PC-3 cells in vivo.The impact of DLL3 on epithelial-mesenchymal transition(EMT)and mitogen-activated protein kinase(MAPK)signaling was investigated through Western blotting.RESULTS:Overexpression of DLL3 significantly enhanced the proliferation,migration,invasion and EMT processes of Pca cells compared with empty vector group and blank control group(P＜0.05).Conversely,knockdown of DLL3 expression yielded opposite effects(P＜0.05).Moreover,up-regulation of DLL3 promoted tumor growth in the nude mouse xenograft model(P＜0.05).Further investigation demonstrated that DLL3 up-regulation led to increases in the levels of MAPK signaling pathway-related proteins.Interestingly,MAPK inhibitor effec-tively reduced the proliferation,migration and invasion of Pca cells caused by DLL3 overexpression(P＜0.05).CON-CLUSION:DLL3 promotes the proliferation,migration and invasion of Pca through the MAPK pathway.
