Simultaneous Determination of 10 Effective Components in the Active Parts of Uygur Medicine Dracocephalum Moldavica L.by HPLC
10.3870/j.issn.1004-0781.2024.04.013
- VernacularTitle:高效液相色谱法同时测定维药香青兰有效部位中10个化学成分的含量
- Author:
Mei'e TAN
1
;
Chenghui HE
;
Hermity PAYMAN
;
Ruifang ZHENG
;
Ning YU
;
Jianguo XING
Author Information
1. 新疆维吾尔自治区药物研究所新疆维吾尔药实验室,乌鲁木齐 830011
- Keywords:
Effective part of Dracocephalum Moldavica L.;
Diosmetin7-O-β-D-glucuronide;
Tilianin;
High performance liquid chromatography(HPLC)
- From:
Herald of Medicine
2024;43(4):567-571
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method for the simultaneous determination of 10 components in the active parts of Uygur medicine Dracocephalum Moldavica L.Methods The determination was performed on a Shim-pack ODS(4.6 mm×250 mm,5 um)column with the mobile phase consisting of acetonitrile(A)-0.5%formic acid(B)in aqueous solution in a gradient elution mode(0-30 min,17%A;30-60 min,17%→ 28%A;60-78 min,28%A)at a flow rate of 1.0 mL·min-1.The temperature of the chromatographic column was 35℃and the detection was monitored by a UV detector at 330 nm.Results Cof-feic acid,p-coumalic acid,cynaroside,luteolin-7-O-β-D-glucuronide,apigenin 7-O-glucuronide,rosmarinic acid,diosmetin7-O-β-D-glucuronide,salvianolic acid A,tilianin,apigenin were well separated under this chromatographic condition,and the linear relation-ship were good in the concentration range examined(r＞0.999 2).The overall recoveries ranged from 91.83%to 106.43%with the RSD ranging from 0.38%to 2.22%.Conclusion The established content determination method is highly accurate and reproduci-ble,and suitable for the analysis and quality control of the active parts of Dracocephalum Moldavica L.
