Effect of Anchusa Italica Retz on Cerebral Ischemia-reperfusion Injury in Rats Based on Disease and Active Compound Target Network
10.3870/j.issn.1004-0781.2024.04.009
- VernacularTitle:基于疾病与活性化合物靶点网络研究牛舌草治疗大鼠脑缺血-再灌注损伤的作用
- Author:
Wenta TAN
1
,
2
;
Bojun HU
;
Bei HUANG
;
Wen ZHANG
;
Shumei WANG
;
Linquan ZANG
;
Guanhua DU
Author Information
1. 广东药科大学药学院,广州 510006
2. 中国医学科学院,北京协和医学院药物研究所,药物靶点研究与新药筛选北京市重点实验室,北京 100050
- Keywords:
Anchusa italica Retz.;
Cerebral ischemia-reperfusion injury;
Network pharmacology
- From:
Herald of Medicine
2024;43(4):535-544
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect and mechanism of Anchusa italica Retz on cerebral ischemia-reperfusion inju-ry in rats based on the target network of active compounds in Anchusa italica Retz.Methods The rat model of cerebral ische-mia-reperfusion injury was established by the thread occlusion method.After performing ischemia for 1.5 h and then reperfusion for 24 h,the neurological function of rats was scored and the volume of cerebral infarction was measured by the 2,3,5-triphenyltet-razolium chloride staining method.The molecular network analysis technique of network pharmacology,protein-protein interaction network,gene ontology(GO)enrichment analysis,KEGG signal pathway analysis,and molecular docking was used to study the mechanism of Anchusa italica Retz in the treatment of cerebral ischemia-reperfusion injury.Results The administration of An-chusa italica Retz could significantly improve the neurobehavioral dysfunction caused by cerebral ischemia-reperfusion injury and reduce the pathological injury of brain tissue.Anchusa italica Retz could regulate inflammation,apoptosis,protein phosphorylation,and other biological processes through 143 ischemic stroke-related targets,and interfere with the TNF signal pathway,VEGF signal pathway,HIF-1 signal pathway,and other pathways.Conclusion Network pharmacology and experimental verification had shown that Anchusa italica Retz could effectively reduce brain injury and protect neurological function through multi-target,multi-mechanism,and holistic treatment of cerebral ischemia-reperfusion injury.
