Mechanism of Essential Oil of Mongolian Medicine Zhenbaowan in the Treatment of Rheumatoid Arthritis Based on GC-MS and Bioinformatics Technology
10.3870/j.issn.1004-0781.2024.04.006
- VernacularTitle:气相色谱-质谱联用结合生物信息分析技术探讨蒙药珍宝丸挥发油治疗类风湿关节炎的作用机制
- Author:
Guangping MA
1
;
Bi QU
;
Ren BU
;
Hua LIAN
;
Wenming BAI
Author Information
1. 内蒙古医科大学 药学院,呼和浩特 010110
- Keywords:
Mongolian medicine Zhenbaowan;
Rheumatoid arthritis;
Bioinformatics;
Gas chromatography-mass spec-trometer;
Antioxidant activity
- From:
Herald of Medicine
2024;43(4):511-519
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the antioxidant capacity of the volatile oil components of the Mongolian medicine Zhenbaowan and the mechanism of action in the treatment of rheumatoid arthritis(RA)by using bioinformatics technology,GC-MS technology,and antioxidant activity experiment.Methods The volatile oil in Zhenbaowan was extracted using water vapor distillation and the volatile oil components were qualitatively analyzed by GC-MS.Based on the results of GC-MS analysis,bioinfor-matics analysis was used to investigate the main components,key targets,and pathways of the volatile oil of Zhenbaowan in pre-venting and treating RA.Meanwhile,the antioxidant activity of the volatile oil was determined and analyzed using the 2,2-Diphen-yl-1-picrylhydrazyl(DPPH)method.Results A total of 115 volatile oil components were identified in Zhenbaowan,of which the top 48 components accounted for 97.66%of the total volatile oil content.Bioinformatics analysis revealed that Zhenbaowan can regulate signal transduction,inflammation,and protein phosphorylation through 147 RA-related targets,and intervene in signaling pathways such as PI3K-Akt,MAPK,and RAS.Molecular docking results showed that the seven main components of Zhenbaowan volatile oil can spontaneously bind to the five core targets.Antioxidant activity tests have proved that the volatile oil of Zhenbaowan has a more significant antioxidant capacity.Conclusion Using GC-MS high-throughput analysis technology combined with bioinformatic analysis and antioxidant activity test,the pharmacodynamic substances and mechanism of action of the volatile oil components of Zhenbaowan,a Mongolian medicine,in the treatment of RA are hypothesized.It provides a theoretical basis for the further study of the volatile oil components of Zhenbaowan.
