Pharmacokinetics of Cordycepin and Its Metabolite 3′-Deoxyinosine in Rats
10.3870/j.issn.1004-0781.2024.03.003
- VernacularTitle:虫草素及其代谢物3′-脱氧肌苷在大鼠体内的药动学
- Author:
Nan HU
1
,
2
;
Zhenwei JIANG
;
Minyan QIAN
;
Wenting ZHANG
;
Lujun CHEN
;
Xiao ZHENG
;
Han-Jie YING
;
Jingting JIANG
Author Information
1. 苏州大学附属第三医院药学部,常州 213000
2. 苏州大学附属第三医院肿瘤生物诊疗中心,常州 213000
- Keywords:
Cordycepin;
3′-deoxyinosine;
Pharmacokinetics;
Liquid chromatography tandem mass spectrometry
- From:
Herald of Medicine
2024;43(3):345-351
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method of LC-MS/MS for determining cordycepin(Cor)and 3′-deoxyinosine(3′-Deo)concentration in rat plasma,and to study their pharmacokinetics in rats.Methods Protein was precipitated with methanol using 2-chloadenosine(2-Chl)as an internal standard.The chromatography was performed on Kinetex C18(3 mm×100 mm,2.6 μm,Phenomenex,USA)with gradient elution in aqueous(5 mmol·L-1 ammonium acetate)-methanol solution as mobile phase.ESI ion source was used for mass spectrometry,and positive ion multiple reaction monitoring(MRM)was used for scanning detection.The pharmacokinetics of Cor and 3′-Deo after oral administration of Cor(10 mg·kg-1)were studied in rats.Results Cor at 0.5-100 ng·mL-1 and 3′-Deo at 1-200 ng·mL-1 had good linearity,and the lower limits of quantification were 0.5 and 1 ng·mL-1,respectively.After oral administration of Cor in rats,the plasma concentration of Cor was low,which was mainly converted into the metabolite 3′-Deo.The Cmax of Cor and 3′-Deo were(5.4±3.4)and(142.0±50.0)ng·mL-1,and AUC0-360min min were(658.4±459.3)and(18 034.9±4 981.1)ng·min·mL-1,respectively.Conclusion The method is simple,sensi-tive,and accurate,which is suitable for determining Cor and 3′-Deo concentration in plasma and the pharmacokinetic study.
