Quality Analysis of Schefflera kwangsiensis Merr.Based on HPLC Fingerprinting Combined with Chemometrics
10.3870/j.issn.1004-0781.2024.02.019
- VernacularTitle:基于高效液相色谱指纹图谱结合化学计量法分析广西鹅掌柴质量
- Author:
Xin YANG
1
;
Nianzhi XU
;
Wenfeng FU
;
Wentao ZHANG
;
Hanzhi YIN
;
Bing LI
Author Information
1. 广西中医药大学药学院,南宁 530200
- Keywords:
Schefflera kwangsiensis Merr;
HPLC fingerprint;
Chemometrics;
Quality analysis
- From:
Herald of Medicine
2024;43(2):267-273
- CountryChina
- Language:Chinese
-
Abstract:
Objective Based on HPLC fingerprinting and chemometrics,to evaluate the quality of Schefflera kwangsiensis Merr.from Guangxi.Methods HPLC was used to establish fingerprints of Schefflera kwangsiensis Merr.from ten different origins,and gradient elution was carried out with methanol-0.1％phosphoric acid aqueous solution as mobile phase.Cluster analysis(CA),principal component analysis(PCA)and orthogonal partial least squares-discriminant analysis(OPLS-DA)were applied to evaluate quality.Results The fingerprints of Schefflera kwangsiensis Merr.from ten different origins were established by HPLC,a total of 22 common peaks were calibrated,with a similarity range of 0.922-0.999.Four chromatographic peaks were identified as rhodopsin,4,5-bis-O-caffeoylquinic acid,caffeic acid,and naringin.The samples were classified into four types according to the CA and OPLS-DA.PCA identified four principal components with a cumulative contribution rare of 95.39％.Conclusion The quality of Schefflera kwangsiensis Merr.can be comprehensively evaluated by fingerprinting combined with CA,PCA and OPLS-DA analysis.The Study can provide a reference for improving the quality control and assessment of Schefflera kwangsiensis Merr.
