Detection and In-house Quality Control Assessment for Cyclosporin A and Tacrolimus by High Performance Liquid Chromatography-tandem Mass Spectormetry Method
10.3870/j.issn.1004-0781.2024.02.008
- VernacularTitle:环孢素A和他克莫司的高效液相色谱-串联质谱检测及其室内质控评价
- Author:
Like ZHONG
1
;
Xiufang MI
;
Qi SHU
;
Gaoqi XU
;
Chaoneng HE
;
Junfeng ZHU
Author Information
1. 浙江省肿瘤医院药剂科,中国科学院杭州医学研究所,杭州 310022
- Keywords:
Cyclosporin A;
Tacrolimus;
High-performance liquid chromatography-tandem mass spectrometry;
Therapeutic drug monitoring;
Westgard multi-rule theory;
Quality control chart
- From:
Herald of Medicine
2024;43(2):196-202
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a quality control method for monitoring the blood concentrations of cyclosporin A and tacrolimus by HPLC-MS/MS,and to evaluate the quality control samples using the Westgard multi-rule theory.Methods HPLC-MS/MS was used to determine the concentration of cyclosporin A and tacrolimus in human whole blood.The quality control samples of low,medium and high concentration levels in the therapeutic drug monitoring process were statistically analyzed,Levery-Jennings and Z-score quality control charts were drawn,and the Westgard multi-rule theory was applied for in-house quality control evaluation.Results The established method was fully validated with linear ranges of 10.40-1 040.00 ng·mL-1 and 0.50-49.50 ng·mL-1,the quantification limits were 10.40 and 0.50 ng·mL-1,respectively.The extraction recoveries were 108.61％-113.24％and 101.99％-109.37％,respectively.The matrix factors normalized by internal standard were 106.68％-111.27％and 95.70％-97.81％for cyclosporin A and tacrolimus,respectively.The intra-day and inter-day accuracy and precision were less than 15.0％.Other parameters were also validated and met the acceptance criteria.Levery-Jennings and Z-score quality control charts showed that there were 4 warnings(violation of the 12s rule)in the results of the 26 groups of quality control samples in the third quarter of 2022,and no phenomenon was observed to be out of control.Conclusion The established in-house quality control system for therapeutic drug monitoring of cyclosporin A and tacrolimus can effectively ensure the accuracy of blood drug concentration detection.
