Effect of Wedelolactone on Oxidative Injury in HUVECs Via the PI3K/Akt/mTOR Signaling Pathway
10.3870/j.issn.1004-0781.2024.02.003
- VernacularTitle:蟛蜞菊内酯通过PI3K/Akt/mTOR信号通路对人脐静脉内皮细胞氧化损伤的影响
- Author:
Sulian LIU
1
;
Kai XIE
;
Dongning YE
;
Wenjing LI
;
Jie CHEN
;
Jing XU
Author Information
1. 南方医科大学皮肤病医院药剂科,广州 510091
- Keywords:
Wedelolactone;
Oxidative stress;
PI3K/Akt/mTOR signaling pathway;
Autophagy;
Human umbilical vein endothelial cells(HUVECs)
- From:
Herald of Medicine
2024;43(2):161-166
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protective effect of Wedelolactone(WEL)against inflammatory injury in human umbilical vein endothelial cells(HUVECs)and its molecular mechanism by inducing PI3K/Akt/mTOR.Methods The model of atherosclerosis(AS)oxidative stress injury in HUVECs was induced with 200 μmol·L-1 of hydrogen peroxide for 24 h.The experimental groups were as follows:normal control group,DMSO(dimethyl sulfoxide)group,H2O2 group,and WEL group.MTT was used to measure the cell survival rate of each group;flow cytometry was used to assess intracellular ROS levels;fluorescence microscopy was used to detect the expression of p62 protein;immunoblotting assay was used to determine the protein expression levels for apoptosis-related proteins associated with PI3K/Akt/mTOR signaling pathway and autophagy-related proteins.Results Compared with the H2 O2 group,the HUVEC cell survival rate was significantly inhibited in the WEL group(P＜0.05).ROS production was significantly lower,and the protein expressions of SOD1 and p62 were significantly increased in the WEL group as compared to the hydrogen peroxide group.The protein expression of p-mTOR,p-Akt,and p-PI3K was significantly decreased in hydrogen peroxide(P＜0.01);In the WEL experiment,p-mTOR,p-Akt,and p-PI3K were increased significantly in the post-injury HUVECs(P＜0.01).Conclusion Wedelolactone inhibits HUVECs'autophagy by suppressing H2O2-induced inflammatory damage in HUVECs,which may be related to the fact that WEL promotes the phosphorylation of PI3K,Akt,and mTOR proteins,inhibits autophagy and thus resists oxidative stress damage in HUVECs cells.
