Determination of Flumatinib and Its Two Major Metabolites in Plasma by LC-MS/MS
10.3870/j.issn.1004-0781.2023.12.006
- VernacularTitle:LC-MS/MS法同时测定血浆中氟马替尼及其代谢产物浓度
- Author:
Yuehua XU
1
;
Zhouyi QIAN
;
Yang ZHAO
;
Qiongye HUANG
;
Luning SUN
;
Yongqing WANG
;
Zhiming SUN
;
Wenwen TANG
Author Information
1. 海安市人民医院药剂科,海安 226600
- Keywords:
Flumatinib;
Liquid chromatography tandem mass spectrometry;
Plasma concentration
- From:
Herald of Medicine
2023;42(12):1779-1784
- CountryChina
- Language:Chinese
-
Abstract:
Objective A simple,specific and rapid LC-MS/MS method was established to determine flumatinib and its two major metabolites in human plasma for clinical therapeutic drug monitoring.Methods The determination was performed on an ACQUITY UPLC HSS T3 column(2.1 mm×50 mm,1.8 μm)with mobile phases consisting of acetonitrile and 10 mmol·L-1 ammonium formate(containing 0.1%formic acid)with gradient elution at the flow rate of 0.5 mL·min-1.The elution time was 6 min.The temperature of the column was 38℃.The ion source was electrospray ion source and the scanning mode was multiple reaction monitoring scanning in positive ion mode.Results The mass concentrations of flumatinib and its metabolites(flumatinib M1 and flumatinib M3)have a good linear relationship within the concentration range investigated.The precision and stability of the method are good.The precision is less than 15%,and the relative deviation is within±15%.The extraction recoveries of flumatinib and its metabolites approach nearly 100%.Conclusion The method is simple and sensitive,and can accurately determine the plasma concentration of flumatinib and its metabolites,providing a basis for clinical rational drug use.
