Histone Acetylation Inhibits the Expression of Asthma Susceptibility Gene ADRB2
10.11969/j.issn.1673-548X.2024.01.025
- VernacularTitle:组蛋白乙酰化抑制哮喘易感基因ADRB2的表达
- Author:
Zhongfen HAO
1
;
Juan DU
;
Yun HANG
Author Information
1. 212001 镇江,江苏大学第四附属医院儿科
- Keywords:
Asthma;
β2-drenergic receptor(ADRB2);
Histone acetylation modification;
BEAS-2B cell
- From:
Journal of Medical Research
2024;53(1):126-130
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of acetylation modification regulated by histone deacetylase inhibitors(HDACi)and his-tone acetyltransferase(HAT)on β2-adrenergic receptor(ADRB2)gene.Methods ADRB2 promoter plasmid(pADRB2-1918)was transfected into BEAS-2B cells,and sodium butyrate(SoB)and trichostatin A(TSA)were added respectively.Adenovirus E1 A-relat-ed kDa protein(p300)plasmid and PADRB2-1918 plasmid were co-transfected into BEAS-2B cells to acetylate histone in BEAS-2B cells.The activity of ADRB2 promoter in BEAS-2B cells treated with SoB,TSA,and p300 was detected by dual-luciferase gene re-porter assay.SoB and TSA were added to BEAS-2B cells,or p300 plasmid was transfected into BEAS-2B cells to acetylate histone in BEAS-2B cells.The relative expression of ADRB2mRNA in BEAS-2B cells was detected by quantitative real-time PCR(qRT-PCR),and the expression of ADRB2 protein in BEAS-2B cells was detected by Western blot.Results SoB,TSA,and p300 plasmid were added into BEAS-2B cells,respectively,which participated in histone acetylation in BEAS-2B cells.We found that the activities of ADRB2 promoter,mRNA,and protein expressions in BEAS-2B cells were lower than those in the control group(P＜0.05),and the differences were statistically significant.Conclusion Histone acetylation can inhibit the expression of ADRB2 in asthma susceptible genes.
