Expression of KIF26B in Bladder Cancer Tissues and its Effect on the Proliferation,Invasion and Migration of Bladder Cancer Cells
10.11969/j.issn.1673-548X.2024.01.022
- VernacularTitle:KIF26B在膀胱癌组织中的表达及对膀胱癌细胞增殖、侵袭、迁移的影响
- Author:
Baode LU
1
;
Xian LONG
;
Yongping HUANG
Author Information
1. 533000 百色,右江民族医学院附属医院泌尿外科
- Keywords:
KIF26B;
Bladder cancer;
Proliferation;
Invasion;
Migration
- From:
Journal of Medical Research
2024;53(1):107-112
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the expression level of kinesin family member 26B(KIF26B)in bladder cancer based on the pub-lic database,and to investigate the effect of silencing KIF26B on the proliferation,invasion and migration of bladder cancer cells.Methods The expression of KIF26B in bladder cancer and its relationship with survival and prognosis were analyzed based on GEO and UaLcan databases.Real-time quantitative polymerase chain reaction(RT-qPCR)and Western blot were used to detect the expression of KIF26B in bladder cancer cell lines(T24,J82)and normal bladder epithelial cells SV-HUV-1.si-KIF26B and si-NC fragments were transfected into T24 cells,and the effects of silencing KIF26B on the proliferation,invasion and migration of T24 cells were detected by methyl thiazolyl tetrazolium(MTT)assay,Transwell assay and scratch assay.Western blot was used to detect the expression levels of p-MEK,MEK,ERK and p-ERK proteins after the silencing of KIF26B.Results KIF26B was highly expressed in bladder cancer tis-sues,and the prognosis of patients with high expression of KIF26B was worse(P＜0.05).The expression level of KIF26B in bladder cancer cell lines T24 and J82 was significantly higher than that in normal bladder epithelial cells SV-HUV-1(P＜0.05).After the si-lencing of KIF26B gene,MTT,Transwell and scratch assay Results showed that the proliferation,invasion and migration ability of T24 cells were significantly decreased(P＜0.05);After silencing KIF26B,the expression of p-MEK and p-ERK proteins in T24 cells was down-regulated(P＜0.05),while MEK and ERK proteins had no significant changes(P＞0.05).Conclusion KIF26B is highly ex-pressed in bladder cancer tissues and cells,which is associated with poor prognosis of patients.Silencing KIF26B can inhibit the prolifera-tion,invasion and migration of bladder cancer cells,and the mechanism may play a role through the MEK/ERK pathway.
