Expression and Correlation of TRPV1/TRPM8 Channels in Intestinal and Spinal Dorsal Root Ganglia in Mice with Experimental Colitis
10.11969/j.issn.1673-548X.2023.12.006
- VernacularTitle:TRPV1、TRPM8通道在实验性结肠炎小鼠肠道和脊髓背根神经节中的表达及相关性研究
- Author:
Lanlan ZHA
1
;
Lei SHEN
;
Jing WU
Author Information
1. 430060 武汉大学人民医院消化内科、消化系统疾病湖北省重点实验室
- Keywords:
Experimental colitis;
Transient receptor potential channel;
Capsaicin receptor;
Inflammatory bowel disease
- From:
Journal of Medical Research
2023;52(12):20-25,88
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of transient receptor potential vanilloid 1(TRPV1)and transient receptor po-tential melastatin member 8(TRPM8)in the intestinal and spinal dorsal root ganglion(DRG)in experimental colitis mice induced by dextran sodium sulfate(DSS),and further explore the connection and related pathways.Methods A total of 20male C57BL/6mice were selected and randomly divided into blank control group and DSS group,with 10mice in each group.Mice in the DSS group were treated with 30g/L DSS for 7days to construct colitis model.The hair,body mass,fecal characteristics(granular,loose stool,bloody stool,etc)and perianal conditions(redness,swelling,bloody stool,etc)of every group were observed and recorded at the same time every day,the colitis related disease activity index(DAI)calculated,and colon histopathological score were calculated according to the pathological sec-tions.The protein expression levels of TRPV1,TRPM8,Gαq in colon tissue were detected by Western blot.The expression levels of in-terleukin-6(IL-6),interleukin-1β(IL-1β)in the colon tissue were detected by real-time quantitative polymerase chain reaction(RT-PCR).The localization and expression of TRPV1,TRPM8,Gαq in colon tissue and DRG were detected by immunofluorescence.Results Compared with the blank control group,the colon pathological damage was obvious in the DSS group,and DAI score of colon tissue was significantly increased(P＜0.05),inflammatory factors including IL-1β,IL-6 and TRPV1,Gαq proteins were up-regula-ted significantly(P＜0.05),TRPM8 expression was down-regulated(P＜0.05).Meanwhile,co-expression of TRPV1/TRPM8,TR-PV 1/Gαq were observed in colonic mucosa and submucosa,and TRPV1/TRPM8 were co-expression in DRG.Compared with the blank control group,TRPV1 expression was increased and TRPM8 was decreased in DSS group.Conclusion The increased TRPV1 expres-sion and decreased TRPM8 expression are found in the colonic tissue of experimental colitis mice,and TRPV1/TRPM8 can be co-expressed in the colonic tissue and DRG.There may be some balance mechanism between them to maintain the stability of intestinal function.
