Bioinformatics Analysis of Cardiac Aging based on GEO Database
10.11969/j.issn.1673-548X.2023.11.010
- VernacularTitle:基于GEO数据库的心脏衰老相关生物信息学分析
- Author:
Yinzhang DING
1
;
Kai WANG
;
Zhongming LI
Author Information
1. 210029 南京医科大学第一附属医院心血管内科
- Keywords:
Cardiac aging;
Bioinformatics;
Differentially expressed genes;
Gene Ontology;
Kyoto Encyclopedia of Genes and Genomes
- From:
Journal of Medical Research
2023;52(11):45-49
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the key genes of cardiac aging by bioinformatic analysis,and conduce to prevent and treat cardio-vascular diseases in the elderly.Methods The gene expression of GSE8146 was downloaded from the Gene Expression Omnibus(GEO)database."R"software was used to screen out differentially expressed genes in the hearts of young and old mice,and DAVID online anal-ysis tool was used for Gene Ontology(GO)function enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)signa-ling pathway enrichment analysis of differentially expressed genes.Hub genes were obtained by protein-protein interaction(PPI)analy-sis based on the STRING online database.Results A total of 55 differentially expressed genes were screened,including 22 up-regula-ted genes and 33down-regulated genes.The differentially expressed genes were significantly enriched in lipid metabolism,fatty acid me-tabolism and other processes,mainly involvded in peroxisome proliferators-activated receptor(PPAR)signaling pathway and Adenosine 5′-monophosphate(AMP)-activated protein kinase(AMPK)signaling pathway.The hub genes such as Fasn,Pck1,Adipoq,Cpt1a,Pdk4,Pnpla2,Slc27a1,Hmgcs2,Cidec,Ucp3 were screened out.Conclusion Cardiac aging may be related to the disorder of cellular energy metabolism,and hub genes such as Fasn,Pck1,Adipoq,Cpt1a,Pdk4,Pnpla2,Slc27a1,Hmgcs2,Ucp3,PPAR signaling path-way and AMPK signaling pathway may play an important role in the development of cardiac aging.
