Clinical Usefulness of Real-time PCR and Amplicor MTB PCR Assays for Diagnosis of Tuberculosis.
10.5145/KJCM.2008.11.1.29
- Author:
Chae Lim JUNG
1
;
Mi Kyung KIM
;
Dong Chun SEO
;
Mi Ae LEE
Author Information
1. Department of Laboratory Medicine, School of Medicine, Ewha Womans University Mokdong Hospital, Seoul, Korea. miae@ewha.ac.kr
- Publication Type:Original Article
- Keywords:
Mycobacterium tuberculosis;
Nontuberculous mycobacteria;
nonrespiratory;
Real- time PCR
- MeSH:
Biological Science Disciplines;
Mycobacterium tuberculosis;
Nontuberculous Mycobacteria;
Polymerase Chain Reaction;
Real-Time Polymerase Chain Reaction;
Sensitivity and Specificity;
Tuberculosis
- From:Korean Journal of Clinical Microbiology
2008;11(1):29-33
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: PCR assay has provided a mean of more rapid and sensitive detection of Mycobacterium tuberculosis (MTB) complex than conventional acid- fast bacilli (AFB) smears and MTB cultures. Using the recently developed AdvanSure TB/NTM kit (LG Life Science Diagnostic Division, Korea), which could differentiate nontuberculous mycobacteria (NTM) from MTB, this study compared clinical usefulness of real-time PCR assay and Amplicor MTB PCR assay (Roche Molecular Systems, USA) for diagnosis of tuberculosis. METHODS: A total of 213 specimens (148 respiratory and 65 nonrespiratory specimens) were tested by using real-time PCR, Amplicor MTB PCR, AFB smear, and MTB culture. The sensitivity and specificity of four methods were evaluated according to clinical diagnosis. RESULTS: Of six NTM grown in culture, four (67%) were detected by real-time PCR. The overall agreement of real-time and Amplicor MTB PCR was 92% (191/207). The overall sensitivity and specificity were 91% and 87%, respectively, for real-time PCR, and 86% and 93% for Amplicor MTB PCR. In nonrespiratory specimens, the sensitivities of real-time PCR, Amplicor MTB PCR, AFB smear, and MTB culture were 67%, 60%, 13%, and 40%, respectively, and the specificity of the four methods were all 100%. CONCLUSION: For diagnosis of tuberculosis, the sensitivity and specificity of the real-time PCR assay using AdvanSure TB/NTM kit and Amplicor MTB PCR were similar, and the former could differentiate NTM from MTB. The PCR assay can be considered as a more sensitive technique for the detection of MTB than the conventional AFB smear and culture.
