Simultaneously quantifying hundreds of acylcarnitines in multiple biological matrices within ten minutes using ultrahigh-performance liquid-chromatography and tandem mass spectrometry
10.1016/j.jpha.2023.10.004
- Author:
Jingxian ZHANG
1
;
Qinsheng CHEN
;
Lianglong ZHANG
;
Biru SHI
;
Men YU
;
Qingxia HUANG
;
Huiru TANG
Author Information
1. State Key Laboratory of Genetic Engineering,School of Life Sciences,Human Phenome Institute,Zhangjiang Fudan International Innovation Center,Metabonomics and Systems Biology Laboratory at Shanghai International Centre for Molecular Phenomics,Zhongshan Hospital,Fudan University,Shanghai,200032,China
- Keywords:
Acylcarnitine;
UPLC-MS/MS;
Quantitative structure-retention;
relationship;
Molecular phenotype
- From:
Journal of Pharmaceutical Analysis
2024;14(1):140-148
- CountryChina
- Language:Chinese
-
Abstract:
Acylcarnitines are metabolic intermediates of fatty acids and branched-chain amino acids having vital biofunctions and pathophysiological significances.Here,we developed a high-throughput method for quantifying hundreds of acylcarnitines in one run using ultrahigh performance liquid chromatography and tandem mass spectrometry(UPLC-MS/MS).This enabled simultaneous quantification of 1136 acyl-carnitines(C0-C26)within 10-min with good sensitivity(limit of detection＜0.7 fmol),linearity(cor-relation coefficient＞0.992),accuracy(relative error＜20％),precision(coefficient of variation(CV),CV＜15％),stability(CV＜15％),and inter-technician consistency(CV＜20％,n=6).We also established a quantitative structure-retention relationship(goodness of fit＞0.998)for predicting retention time(tR)of acylcarnitines with no standards and built a database of their multiple reaction monitoring parameters(tR,ion-pairs,and collision energy).Furthermore,we quantified 514 acylcarnitines in human plasma and urine,mouse kidney,liver,heart,lung,and muscle.This provides a rapid method for quantifying acyl-carnitines in multiple biological matrices.
