Polarity-regulated derivatization-assisted LC-MS method for amino-containing metabolites profiling in gastric cancer
10.1016/j.jpha.2023.06.009
- Author:
Jie HAN
1
;
Shilin GONG
;
Xiqing BIAN
;
Yun QIAN
;
Guilan WANG
;
Na LI
;
Jian-Lin WU
Author Information
1. State Key Laboratory of Quality Research in Chinese Medicine,Macau Institute for Applied Research in Medicine and Health,Macau University of Science and Technology,Avenida Wai Long,Taipa,Macau,999078,China
- Keywords:
Polarity-regulated derivatization;
Amino-containing metabolites;
Gastric cancer;
Liquid chromatography-mass spectrometry
- From:
Journal of Pharmaceutical Analysis
2023;13(11):1353-1364
- CountryChina
- Language:Chinese
-
Abstract:
Amino-containing compounds,including amino acids,aliphatic amines,aromatic amines,small peptides and catecholamines,are involved in various biological processes and play vital roles in multiple meta-bolic pathways.Previous studies indicated that some amino-containing metabolites are significant diagnostic and prognostic biomarkers of gastric cancer.However,the discovery of precise biomarkers for the preoperative diagnosis of gastric cancer is still in an urgent need.Herein,we established a polarity-regulated derivatization method coupled with liquid chromatography-mass spectrometry(LC-MS)for amino-containing metabolites profiling in the serum samples of patients with gastric cancer and healthy controls,based on our newly designed and synthesized derivatization reagent(S)-3-(1-(diisopropox-yphosphoryl)pyrrolidine-2-carboxamido)-N-hydroxysuccinimidyl ester(3-DP-NHS).Enhanced separa-tion efficiency and detection sensitivity for amino-containing metabolites were achieved after derivatization.This method exhibited good linearity,recovery,intra-and inter-day precision and accu-racy.Only 5 μL serum is needed for untargeted analysis,enabling 202 amino-containing metabolites to be detected.Statistical analysis revealed altered amino acid metabolisms in patients with gastric cancer.Furthermore,ultra high performance liquid chromatography coupled with mass spectrometry(UHPLC-MS/MS)analysis quantification revealed increased serum levels of tryptamine and decreased concen-trations of arginine and tryptophan in patients with gastric cancer.Receiver operating characteristic(ROC)curves indicated that an increased tryptamine/tryptophan ratio could serve as a potential biomarker for gastric cancer diagnosis.This study demostrated the possibility of using serum amino acid biomarkers for gastric cancer diagnosis,providing new avenues for the treatment of gastric cancer.
