Characterization of natural peptides in Pheretima by integrating proteogenomics and label-free peptidomics
10.1016/j.jpha.2023.06.006
- Author:
Xiaoxiao LUO
1
,
2
;
Qirui BI
;
Dongdong HUANG
;
Yun LI
;
Changliang YAO
;
Jianqing ZHANG
;
Wenlong WEI
;
Jiayuan LI
;
Zhenwei LI
;
Jingxian ZHANG
;
Shen JI
;
Yurong WANG
;
De-An GUO
Author Information
1. School of Basic Medicine and Clinical Pharmacy,China Pharmaceutical University,Nanjing,211198,China
2. Shanghai Research Center for Modernization of Traditional Chinese Medicine,National Engineering Research Center of TCM Standardization Technology,Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai,201203,China
- Keywords:
Pheretima;
Natural peptides;
Label-free peptidomics;
Proteogenomics;
Animal derived medicine
- From:
Journal of Pharmaceutical Analysis
2023;13(9):1070-1079
- CountryChina
- Language:Chinese
-
Abstract:
Pheretima,also called"earthworms",is a well-known animal-derived traditional Chinese medicine that is extensively used in over 50 Chinese patent medicines(CPMs)in Chinese Pharmacopoeia(2020 edi-tion).However,its zoological origin is unclear,both in the herbal market and CPMs.In this study,a strategy for integrating in-house annotated protein databases constructed from close evolutionary relationship-sourced RNA sequencing data from public archival resources and various sequencing al-gorithms(restricted search,open search,and de novo)was developed to characterize the phenotype of natural peptides of three major commercial species of Pheretima,including Pheretima aspergillum(PA),Pheretima vulgaris(PV),and Metaphire magna(MM).We identified 10,477 natural peptides in the PA,7,451 in PV,and 5,896 in MM samples.Five specific signature peptides were screened and then validated using synthetic peptides;these demonstrated robust specificity for the authentication of PA,PV,and MM.Finally,all marker peptides were successfully applied to identify the zoological origins of Brain Heart capsules and Xiaohuoluo pills,revealing the inconsistent Pheretima species used in these CPMs.In conclusion,our integrated strategy could be used for the in-depth characterization of natural peptides of other animal-derived traditional Chinese medicines,especially non-model species with poorly annotated protein databases.
