Ultrasensitive proteomics depicted an in-depth landscape for the very early stage of mouse maternal-to-zygotic transition
10.1016/j.jpha.2023.05.003
- Author:
Lei GU
1
;
Xumiao LI
;
Wencheng ZHU
;
Yi SHEN
;
Qinqin WANG
;
Wenjun LIU
;
Junfeng ZHANG
;
Huiping ZHANG
;
Jingquan LI
;
Ziyi LI
;
Zhen LIU
;
Chen LI
;
Hui WANG
Author Information
1. Center for Single-Cell Omics,School of Public Health,Shanghai Jiao Tong University School of Medicine,Shanghai,200025,China
- Keywords:
Single-cell proteomics;
Low-input proteomics;
Maternal-to-zygotic transition;
Oocyte;
Embryo
- From:
Journal of Pharmaceutical Analysis
2023;13(8):942-954
- CountryChina
- Language:Chinese
-
Abstract:
Single-cell or low-input multi-omics techniques have revolutionized the study of pre-implantation embryo development.However,the single-cell or low-input proteomic research in this field is rela-tively underdeveloped because of the higher threshold of the starting material for mammalian embryo samples and the lack of hypersensitive proteome technology.In this study,a comprehensive solution of ultrasensitive proteome technology(CS-UPT)was developed for single-cell or low-input mouse oocyte/embryo samples.The deep coverage and high-throughput routes significantly reduced the starting material and were selected by investigators based on their demands.Using the deep coverage route,we provided the first large-scale snapshot of the very early stage of mouse maternal-to-zygotic transition,including almost 5,500 protein groups from 20 mouse oocytes or zygotes for each sample.Moreover,significant protein regulatory networks centered on transcription factors and kinases between the MII oocyte and 1-cell embryo provided rich insights into minor zygotic genome activation.
