Cux1+ proliferative basal cells promote epidermal hyperplasia in chronic dry skin disease identified by single-cell RNA transcriptomics
10.1016/j.jpha.2023.04.004
- Author:
Minhua HUANG
1
;
Ning HUA
;
Siyi ZHUANG
;
Qiuyuan FANG
;
Jiangming SHANG
;
Zhen WANG
;
Xiaohua TAO
;
Jianguo NIU
;
Xiangyao LI
;
Peilin YU
;
Wei YANG
Author Information
1. Department of Biophysics,and Department of Neurology of the Fourth Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou,310000,China
- Keywords:
scRNA-seq;
Dry skin;
Proliferative basal cells
- From:
Journal of Pharmaceutical Analysis
2023;13(7):745-759
- CountryChina
- Language:Chinese
-
Abstract:
Pathological dry skin is a disturbing and intractable healthcare burden,characterized by epithelial hy-perplasia and severe itch.Atopic dermatitis(AD)and psoriasis models with complications of dry skin have been studied using single-cell RNA sequencing(scRNA-seq).However,scRNA-seq analysis of the dry skin mouse model(acetone/ether/water(AEW)-treated model)is still lacking.Here,we used scRNA-seq and in situ hybridization to identify a novel proliferative basal cell(PBC)state that exclusively expresses transcription factor CUT-like homeobox 1(Cux1).Further in vitro study demonstrated that Cux1 is vital for keratinocyte proliferation by regulating a series of cyclin-dependent kinases(CDKs)and cyclins.Clinically,Cux1+PBCs were increased in patients with psoriasis,suggesting that Cux1+ PBCs play an important part in epidermal hyperplasia.This study presents a systematic knowledge of the tran-scriptomic changes in a chronic dry skin mouse model,as well as a potential therapeutic target against dry skin-related dermatoses.
