Clinical value of metagenomics next-generation sequencing in the diagnosis of urinary calculi with secondary infection
10.3760/cma.j.cn115455-20221123-01024
- VernacularTitle:宏基因组学二代测序技术对尿路结石继发感染的诊断价值
- Author:
Zhongliang HONG
1
;
Mingfa WAN
;
Yizhou YE
;
Zewei SUN
;
Qian XU
Author Information
1. 浙江省桐庐县第一人民医院泌尿外科，杭州 311500
- Keywords:
Urinary calculi;
Infection;
Metagenomics next-generation sequencing;
Pathogen;
Diagnosis
- From:
Chinese Journal of Postgraduates of Medicine
2023;46(12):1144-1148
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the clinical value of metagenomics next-generation sequencing (mNGS) in the diagnosis of urinary calculi with secondary infection.Methods:From September 2021 to May 2022, a total of 110 urinary calculi patients from the First People′s Hospital of Zhejiang Province Tonglu County were collected retrospectively, the urine sample of the patients with bacterial meningitis was measured by urine bacterial culture and mNGS respectively. Taking urine bacterial culture as the "gold standard", the sensitivity, specificity, positive predictive value, negative predictive value, and Kappa consistency of mNGS in the diagnosing of urinary calculi with secondary infection were analyzed. Results:The positive of urine bacterial culture were 35 cases and negative were 75 cases; while positive and negative were 39 cases and 71 cases in the mNGS detection. Taking urinary bacterial culture as the "gold standard", the specificity, sensitivity, positive predictive value, negative predictive value and Kappa consistency coefficient of mNGS in the diagnosis of secondary infection of urinary calculi were 89.3%, 88.6%, 79.5%, 94.4% and 0.756 respectively. Compared with urine bacterial culture, the Kappa consistency coefficients of three common pathogens detected by the mNGS of macrogenomics, included escherichia coli, klebsiella pneumoniae and enterococcus faecalis were 0.703, 0.735 and 0.769, respectively. Conclusions:mNGS can improve the detection rate of pathogens of secondary infection of urinary calculi, and has a high consistency with the detection results of urinary bacterial culture.
