Research progress in SARS-CoV-2 nucleic acid detection based on microfluidic platforms
10.19745/j.1003-8868.2024017
- VernacularTitle:基于微流控平台的SARS-CoV-2核酸检测的研究进展
- Author:
Fan YANG
1
;
Lan WANG
;
Hong QIU
;
Cheng KONG
;
Wei-Wei ZHANG
;
Chang GU
;
Yue-Rong ZHU
Author Information
1. 东部战区总医院检验科,南京 210002
- Keywords:
microfluidics;
SARS-CoV-2;
nucleic acid detection;
RT-qPCR;
digital PCR;
CRISPR/Cas
- From:
Chinese Medical Equipment Journal
2024;45(1):101-107
- CountryChina
- Language:Chinese
-
Abstract:
The detection principle of microfluidic microfluidic technology was introduced.The current research status of microfluidic platform-based SARS-CoV-2 nucleic acid detection technologies were reviewed such as reverse transcription quantitative real-time polymerase chain reaction(RT-qPCR),digital PCR,isothermal amplification and clustered regularly interspaced palindromic repeats/CRISPR-associated protein.The deficiencies of microfluidic platform-based SARS-CoV-2 nucleic acid detection were analyzed.It's pointed out microfluidic platform-based SARS-CoV-2 nucleic acid detection had to be optimized and validated clinically in specialty,sensitivity,detection limit,reproducibility,informatization,quality control and reagent cost.[Chinese Medical Equipment Journal,2024,45(1):101-107]
