Construction and performance evaluation of ultra-fast thermal cycle fluorescence quantitative PCR system
10.19745/j.1003-8868.2023222
- VernacularTitle:极速热循环荧光定量PCR系统的设计及性能评估
- Author:
Hai-Yan ZHANG
1
;
Qi-Yue CHEN
;
Zhong-Ming LIU
;
Jing DENG
;
Yan-Tong GUO
;
Xiao-Ming ZHANG
Author Information
1. 南部战区总医院,广州 510010
- Keywords:
ultra-fast thermal cycle;
fluorescence quantification;
PCR system;
influenza virus;
SARS-CoV-2
- From:
Chinese Medical Equipment Journal
2023;44(11):15-20
- CountryChina
- Language:Chinese
-
Abstract:
Objective To design an ultra-fast thermal cycle fluorescent quantitative PCR system for on-site detection of pathogenic nucleic acids and evaluate its performances.Methods An ultra-fast thermal cycle fluorescent quantitative PCR system was developed with the components of a flat reaction cup,an ultra-fast thermal cycle module,a fluorescence detection module with fixed optical path and a data processing module based on the smartphone platform.The ultra-fast thermal cycle module was composed of a heating unit and a cooling unit,of which the heating unit was made of ceramic sheet and Ag/Pb alloy and the cooling unit consisted of a high-speed magnetic levitation cooling fan and a double-curved throat;the fluorescence detection module with fixed optical path was prepared with injection molding process,and made up of a light source excitation unit and a light detector unit;the data processing module based on the smartphone platform included a Bluetooth serial port adapter unit and a smartphone App,which used C2540F256 chip from TI company for developing the Bluetooth serial port adapter and Android Studio for the App.The ultra-fast thermal cycle fluorescent quantitative PCR system was used to detect influenza A/B virus and SARS-CoV-2 to verify its performances.Results The ultra-fast thermal cycle fluorescent quantitative PCR system realized rapid nucleic acid detection of influenza A/B virus and SARS-CoV-2,and the detection results were in high agreement with those by conventional real-time quantitative PCR.Conclusion The ultra-fast thermal cycle fluorescence quantitative PCR system gains advantages in small size and light weight,and can be used for rapid on-site detection of pathogen nucleic acids.[Chinese Medical Equipment Journal,2023,44(11):15-20]
