Research progress on oral mucosal epithelial culture and ocular surface transplantation
10.3760/cma.j.cn115989-20200304-00142
- VernacularTitle:口腔黏膜上皮培养与眼表移植的研究进展
- Author:
Danni GONG
1
;
Chenxi YAN
;
Yao FU
Author Information
1. 上海交通大学医学院附属第九人民医院眼科 上海市眼眶病眼肿瘤重点实验室，上海 200011
- Keywords:
Oral mucosal epithelial cells;
Culture in vitro;
Limbal stem cell deficiency;
Ocular surface reconstruction
- From:
Chinese Journal of Experimental Ophthalmology
2023;41(11):1135-1139
- CountryChina
- Language:Chinese
-
Abstract:
Human corneal epithelial cells are continuously replenished by limbal stem cells to maintain ocular homeostasis and normal visual function.Some ocular surface injuries or diseases, such as thermochemical burns and Stevens-Johnson syndrome, can result in limbal stem cell deficiency, severely compromising patients' visual acuity.Non-limbal autologous epithelial tissue transplantation should be considered when both eyes are affected.Oral mucosal epithelial cells are an important source of seed cells for ocular surface reconstruction.What's more, cell sheets constructed ex vivo have achieved good results in clinical applications.This article reviews the research progress on the use of oral mucosal epithelial cells for the treatment of limbal stem cell deficiency, focusing on various factors including carriers, culture conditions, and techniques that may affect the culture of oral mucosal epithelial sheets.In addition, the strengths and weaknesses of the application of cell sheets in clinical ocular surface transplantation are also discussed, so as to provide a research direction for the safe and effective reconstruction of ocular surface epithelium.
