Analysisof drug resistance mechanism and characteristics of carbon-resistant pseudomonas aeruginosa
10.3969/j.issn.1671-332X.2024.01.038
- VernacularTitle:耐碳青霉烯类铜绿假单胞菌的耐药机制及特征分析
- Author:
Yaping WANG
1
;
Julaiti MAIWULIDAN
;
Zhongwen ZHANG
;
Yuzhen LI
;
Lingqing XU
;
Weihong WEN
Author Information
1. 广州医科大学附属第六医院//清远市人民医院 广东清远 511518
- Keywords:
Pseudomonas aeruginosa;
Carbapenem;
Cephalosporin/avebatan;
Drug resistance characteristics
- From:Modern Hospital
2024;24(1):144-148
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the drug resistance mechanism and characteristics of carbopenem-resistant pseudo-monas aeruginosa(CRPA)in our hospital.Methods BD phoenix 100 automatic bacterial identification and drug sensitivity an-alyzer was used to identify and detect the drug sensitivity of the strains.The minimum inhibitory concentration(MIC)of ceftazi-dime/acibactam was detected by micro broth dilution method.The modified carbapenem inactivation method(mCIM)and colloi-dal gold immunochromatography were used to detect the carbapenemase phenotype of the strains.The whole genome sequencing was used to detect the carbapenemase resistance gene and ST typing of the screened positive strains.Results A total of 22 strains of clinically isolated CRPA were collected,of which the antibacterial drugs with the lowest resistance rate were ceftidine/avibatan(22.7% ),followed by gentamicin and amikacin(27.3% ),pyracillin/tazobactam(59.09% ),cefuroxime(63.6% ).Ceftazide and aminotransferrane(77.27% ),ciprofloxacin(86.36% ),levofloxacin(95.45% ).There are a total of 5 strains(22.7% )of carbapenems in 22 CRPA by phenotypic detection.The whole genome sequencing results show that 4 strains of ST549 CRPA carry metal β-lactamase IMP-45 and serine β-lactamase OXA-1,OXA-50,one strain is ST245 CRPA carries metal β-lactamase NDM-1,that is,all five CRPA strains produce metal β-lactamase.Conclusion The resistance rate of CRPA to ceftazidime/avibactam is low in our hospital.Carbapenemase-producing is not the main mechanism of CRPA resistance to car-bapenems,while metal β-Lactamase-producing is the main mechanism of CRPA resistance to ceftazidime avibactam.
