Simplified EM Grid Vitrification Is a Convenient and Efficient Method for Mouse Mature Oocyte Cryopreservation.
10.3349/ymj.2006.47.3.399
- Author:
Seok Hyun KIM
1
;
Seung Yup KU
;
Ki Cheong SUNG
;
Moon Joo KANG
;
Sung Ah KIM
;
Hee Sun KIM
;
Sun Kyung OH
;
Byung Chul JEE
;
Chang Suk SUH
;
Young Min CHOI
;
Jung Gu KIM
;
Shin Yong MOON
Author Information
1. Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, Korea. shmoon@plaza.snu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
EM grid vitrification;
cryopreservation;
mouse oocyte
- MeSH:
Pregnancy;
Oocytes/*cytology;
Mice, Inbred DBA;
Mice, Inbred C57BL;
Mice;
Male;
*Fertilization in Vitro;
Female;
Cryopreservation/*instrumentation/*methods;
Cell Survival;
Animals
- From:Yonsei Medical Journal
2006;47(3):399-404
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study was performed to evaluate the efficiency of simplified EM grid vitrification, skipping the step of removing the cryoprotectant (5.5M EG + 1.0M sucrose) droplet on the grid after loading oocytes, compared to conventional cryopreservation protocols for mouse mature oocytes. Firstly, the recovery, survival, fertilization and hatching rates of simplified EM grid vitrification were compared with those of the slow freezing method using 1.5M DMSO. Then, conventional EM grid vitrification was compared with simplified EM grid vitrification. Simplified EM grid vitrification showed higher survival, fertilization and hatching rates than those of the slow freezing method (85.6% vs. 63.2%; 51.0% vs. 22.3%; 38.7% vs. 12.5%, p < 0.01, respectively). Moreover, simplified EM grid vitrification showed higher recovery, survival and fertilization rates than those of conventional EM grid vitrification (100% vs. 95.0%, p=0.024; 90.0% vs. 78.9%, p=0.033; 56.7% vs. 38.7%, p=0.021, respectively). Hatching rate tended to be higher for simplified EM grid vitrification compared to conventional EM grid vitrification (41.1% vs. 24.1%). In conclusion, simplified EM grid vitrification is a convenient and efficient method for cryopreservation of mouse mature oocytes, compared to conventional EM grid vitrification and slow freezing methods.
