The construction of rat intestinal smooth muscle collagen band and evaluation of periodic stretching culture in vitro
10.12307/2024.806
- VernacularTitle:大鼠肠道平滑肌胶原条带构建及周期性拉伸培养的体外评价
- Author:
Pengxin YU
1
;
Yuqiu HAN
;
Lina GUO
;
Xiuli WANG
Author Information
1. 大连医科大学,辽宁省大连市 116000
- Keywords:
tissue engineering;
intestinal smooth muscle;
collagen band;
dynamic stretching;
collagen scaffold;
three-dimensional culture
- From:
Chinese Journal of Tissue Engineering Research
2024;28(35):5630-5635
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:The in vitro construction of intestinal smooth muscle layer,as an important component of the intestinal wall,has attracted much attention in the bionic construction of tissue-engineered intestinal canal. OBJECTIVE:To explore the effects of cyclic mechanical stretching on the growth activity of intestinal smooth muscle cells and the expression of functional genes within collagen strips. METHODS:The collagen band culture system of intestinal smooth muscle cells was constructed using a self-designed collagen strip stretching culture device with self-made rat tail collagen as a scaffold and primary rat intestinal smooth muscle cells as seed cells.EthD-1/Calcein-AM cell activity staining,magenta staining,cytoskeleton-Ki67 immunofluorescence staining were used to observe the growth activity and proliferation of the cells,and quantitative RT-PCR was used to detect the expression of desmin,α-sma,and vimentin functional genes. RESULTS AND CONCLUSION:The collagen band culture system of intestinal smooth muscle cells was successfully constructed,and intestinal smooth muscle cells in the band had good cell activity.The number of Ki67 positive cells increased and desmin,α-sma and vimentin were significantly overexpressed under cyclic stretching and dynamic culture conditions(P＜0.001).To conclude,mechanical stimulation is beneficial to maintain the growth phenotype of smooth muscle cells and promote their functional differentiation during three-dimensional culture in vitro.
