Effect of Progesterone on COX-2 Expression and Proliferation of Prostate Stromal Cell.
10.12701/yujm.2006.23.1.62
- Author:
Soo Ryun JUNG
1
;
Sung Han KIM
;
E Hwa CHOI
;
Ji Eun PARK
;
Eun Mi JEON
;
Young Jin KANG
;
Kwang Youn LEE
;
Hyoung Chul CHOI
Author Information
1. Senior grade, College of Medicine, Yeungnam University, Daegu, Korea.
- Publication Type:Original Article
- Keywords:
Prostate stromal cell;
Progesterone;
Cyclooxygenase-2
- MeSH:
Cell Count;
Cyclooxygenase 2;
Dihydrotestosterone;
Humans;
Male;
Progesterone*;
Prostate*;
Prostatic Hyperplasia;
Receptors, Androgen;
RNA, Messenger;
Stromal Cells*;
Testosterone
- From:Yeungnam University Journal of Medicine
2006;23(1):62-70
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Benign prostatic hyperplasia (BPH) is the most common benign tumor in older men; the etiology of this disease remains poorly understood. Testosterone and dihydrotestosterone (DHT) both act as androgen via a single androgen receptor. Testosterone is converted to DHT by 5alpha-reductase in prostatic stromal cells. Progesterone has been reported to inhibit DHT conversion; howevwe, its effect on prostatic stromal cells remains to be elucidated. MATERILAS AND METHODS: In this experiment, we investigated the effect of progesterone on androgen receptor expression induced by DHT. We also tested the effect of progesterone on cyclooxygenase-2 (COX-2) expression, as well as prostate stromal cell proliferation using the cell count kit-8. RESULTS: Progesterone did not cause an increase of prostate stromal cell proliferation. The mRNA expression of the androgen receptor and COX-2 were not changed by progesterone; the expressions of androgen receptor and COX-2 proteins were decreased by progesterone in prostate stromal cells. CONCLUSION: These results suggest that in prostate stromal cells, progesterone decreases androgen receptor protein expression, which results in decrement of COX-2 protein expression. This effect might be mediated by post-transcriptional regulation.
