Analysis of Matrix Metalloproteinase-9 Expression in Renal Cell Carcinoma.
10.12701/yujm.2006.23.1.82
- Author:
Ji Yoon KIM
1
;
Tong Choon PARK
Author Information
1. Department of Urology, College of Medicine, Yeungnam University, Daegu, Korea. tcpark@med.yu.ac.kr
- Publication Type:Original Article
- Keywords:
Matrix metalloproteinases;
Renal cell carcinoma
- MeSH:
Carcinogenesis;
Carcinoma, Renal Cell*;
Extracellular Matrix;
Gelatin;
Humans;
Kidney;
Matrix Metalloproteinase 9*;
Matrix Metalloproteinases;
Neoplasm Metastasis;
Nephrectomy
- From:Yeungnam University Journal of Medicine
2006;23(1):82-89
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Matrix metalloproteinases (MMPs) are involved in the degradation of the extracellular matrix, which is an important step in tumor invasion and metastasis. A positive correlation between the expression of MMP-9 and aggressive behavior of renal cell carcinomas (RCCs) has been reported. MMP-9 expression in RCCs and adjacent normal kidney tissues were examined in this study. MATERILAS AND METHODS: Twenty-five patients pathologically diagnosed as clear cell RCCs, from specimens obtained at radical nephrectomy, between May 2003 and December 2004 were enrolled in this study. MMP-9 activity was estimated using gelatin zymography, and quantified using a laser densitometer. The results were compared with clinicopathological characteristics. RESULTS: The expression of MMP-9 was significantly elevated in the RCC compared with non-tumor kidney specimens (p<0.01). The levels of MMP-9 expression in the RCC patients with large tumors (>4 cm) or vascular invasion were significantly higher than in those without these clinical manifestations (p<0.01). There were also significant differences in the expression of MMP-9 among T stages (p<0.01). The tissue MMP-9 level was the highest in nuclear grade 4, but there was no statistical significance between the histological grades (p=0.17). CONCLUSIONS: These results suggest that enhanced MMP-9 expression contributes to carcinogenesis and tumor progression in the later stages of RCC.
