Removal of the selective marker gene in the fusion gene expression vector of plant anti-caries vaccine

Yaoling Lang; Qian Wang; Bin Chen; Guohui Bai; Xiaoyan Guan; Jianguo Liu

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Removal of the selective marker gene in the fusion gene expression vector of plant anti-caries vaccine

VernacularTitle:植物防龋疫苗融合基因表达载体中选择标记基因的去除
Author: Yaoling LANG ^{1
,

2} ; Qian WANG ; Bin CHEN ; Guohui BAI ; Xiaoyan GUAN ; Jianguo LIU
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1. 贵州省普通高等学校口腔疾病研究特色重点实验室暨遵义市口腔疾病研究重点实验室,遵义医科大学口腔医学院,贵州省遵义市 563000
2. 贵阳市口腔医院,贵州省贵阳市 550002
Keywords: plant vaccine; immune anti-caries; expression vector; marker gene; transgenic safety; E8 promoter
From: Chinese Journal of Tissue Engineering Research 2024;28(1):7-11
CountryChina
Language:Chinese
Abstract: BACKGROUND:In consideration of the food safety and ecological safety of transgenic plants,the retention of marker genes is the primary safety issue affecting transgenic plants. OBJECTIVE:Based on the principle of immune caries prevention,our research team successfully constructed the plant anti-caries vaccine fusion gene expression vector pCAMBIA-E8-APB-DOCK8 for these two caries causing virulence factors surface protein and glucosyltransferase,which provides a basis for the research and development of transgenic plant vaccine. METHODS:In this study,the selective marker genes Km and GUS in the plant caries vaccine fusion gene expression vector pCAMBIA-E8-APB-DOCK8 were removed by DNA recombination technology through a series of steps such as DNA fragment separation,connection,transformation,clone detection,and sequencing. RESULTS AND CONCLUSION:The efficiency of marker gene removal was 99%.This study has laid a good experimental foundation for the safe production of transgenic plant vaccine against dental caries,and also provided ideas for the construction of other plant vaccine vectors.