Linc-smad7 promotes migration and invasion of pancreatic cancer cells by targeting miR-125b/SIRT1 axis
- VernacularTitle:Linc-smad7通过靶向miR-125b/SIRT1轴促进胰腺癌细胞迁移和侵袭
- Author:
Lili HAN
1
;
Wenjiao DUAN
;
Weixiao ZHOU
;
Shuqun ZHANG
Author Information
- Keywords: pancreatic cancer; Linc-smad7; miR-125b; migration; invasion
- From: Journal of Xi'an Jiaotong University(Medical Sciences) 2024;45(1):112-118
- CountryChina
- Language:Chinese
- Abstract: Objective To explore the role of long non-coding RNA smad7(Linc-smad7)in invasion and migration of pancreatic cancer(PC)cells and its mechanisms.Methods The expression level of Linc-smad7 in PC tissues and cell lines was detected by qRT-PCR assays.Transwell assays were performed to observe the effect of Linc-smad7 overexpression on the migration and invasion abilities of PaCa-2 cells.Luciferase reporter analysis was made to detect the direct binding effect of Linc-smad7 on miR-125b and miR-125b on Sirtuin1(SIRT1).qRT-PCR assays were used to detect the regulatory effect of Linc-smad7 on miR-125b expression in PaCa-2 cells.qRT-PCR and Western blotting assays were used to detect the regulatory effect of miR-125b on SIRT1 expression.Results Linc-smad7 was significantly increased in PC tissues and cell lines.Paca-2 cells with overexpressed Linc-smad7 showed higher migration and invasion abilities,while miR-125b expression was decreased.After the expression of miR-125b was increased,the expression of SIRT1 was decreased significantly.Luciferase reporter assays results suggested that Linc-smad7 directly targeted with miR-125b,and miR-125b directly bound with SIRT1.Increased Linc-Smad7 or decreased miR-125b could reverse the effect of SIRT1 inhibition on invasion and migration of PaCa-2 cells.Conclusion Linc-smad7 promotes invasion and migration of PaCa-2 cells through miR-125b/SIRT1 axis.
