Study on the mechanism of genistein inhibiting the progression and metastasis of prostate cancer
- VernacularTitle:染料木黄酮抑制前列腺癌进展和转移的机制研究
- Author:
Wenzhan LIU
1
;
Qiliang CAI
;
Baojun WU
;
Siwei YANG
;
Zhili YAO
;
Zekai HOU
;
Binxu SUN
Author Information
- Keywords: prostatic neoplasms; genistein; epithelial-mesenchymal transition; cell movement
- From: Tianjin Medical Journal 2023;51(12):1288-1292
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the effect of genistein on the proliferation,migration and invasion of prostate cancer cells and its molecular mechanism.Methods Prostate cancer LNCaP and CWR22RV1 cells were divided into the control group(conventional culture)and the experimental group(50μmol/L genistein treatment).The effect of genistein on the proliferation of prostate cancer cells were analyzed by MTT assay.The effect of genistein on the migration and invasion of prostate cancer cells were analyzed by cell scratch assay and Transwell assay.The protein levels of epithelial interstital transformation(EMT)intermediate markers E-Cadherin,N-Cadherin,Vimentin,and tumor stem cell markers CD44 and Oct-4 were detected by Western blot assay.Results MTT assay showed that genistein could inhibit the proliferation of prostate cancer cells.The scratch closure rates of LNCaP and CWR22RV1 cells were significantly reduced in the experimental group compared with those in the control group,and the number of cells passing through the Transwell membrane was significantly reduced(P＜0.05).Western blot assay showed that genistein could down-regulate the expression levels of N-Cadherin,Vimentin,CD44 and Oct4 in prostate cancer cells,and up-regulate the expression of E-Cadherin in epithelial cells(P＜0.01).Conclusion Genistein reduces the dryness of prostate cancer cells by inhibiting the EMT process,thus reducing the proliferation,migration and invasion of prostate cancer cells.
