LAMP3 inhibited the proliferation,metastatic and PC-3-induced vasculogenesis of HUVEC by regulating VEGF/AKT signaling
10.3969/j.issn.1006-5725.2024.02.010
- VernacularTitle:溶酶体相关膜蛋白3通过VEGF/AKT通路抑制PC-3细胞增殖、转移及血管生成
- Author:
Canwei CHEN
1
;
Zhuangwen LIAO
;
Ziwen FAN
;
Shuai HUANG
;
Yan HUANG
;
Binwei CHEN
Author Information
1. 广州医科大学附属第二医院骨科(广州 510260)
- Keywords:
lysosome-associated membrane protein 3;
prostate cancer;
bone metastasis;
vascular endothelial growth factor;
angiogenesis
- From:
The Journal of Practical Medicine
2024;40(2):182-187
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the impactof Lysosome-Associated Membrane Protein 3(LAMP3)on theproliferation,migration and angiogenesis of PC-3 cells.Methods LAMP3 expression in normal prostate epithelial cells and prostate cancer bone metastasis cells was detected using western blot and RT-PCR.Stable LAMP3-silenced PC-3 cells were constructed,and the effects of LAMP3 on proliferation,invasion,and migration of PC-3 cells were assessed using CCK8,scratch assay,and transwell assay,respectively.ELISA and angiogenesis assays were employed to examine the expression of VEGF and MMP9,as well as angiogenesis of HUVEC cells induced by PC-3 cells.Finally,WB and RT-PCR were used to detect the expression of VEGF,AKT/p-AKT.Results Our findings showed that the expression level of LAMP3 was significantly higherin prostate cellsthan in normal prostate epithelial cells,especially in PC-3 cells(P<0.05).We also found that silencing LAMP3 could inhibit the proliferation,migration and invasion of PC-3 cells,along with the expression of VEGF and MMP9 and the PC-3 cells-induced angiogenesis,and these results were statistically significant(P<0.05).Furthermore,LAMP3 downregulated the expression of VEGF and AKT/p-AKT in PC-3 cells.Conclusion LAMP3 can affect the proliferation,migrationand angiogenesis of PC-3 cells through the regulation of VEGF/AKT pathway.Thus,LAMP3 might be a potential thera-peutic target for prostate cancer bone metastasis.
