Levels and significance of γδ T cells and their subpopulations in the bone marrow of MDS patients
10.3969/j.issn.1006-5725.2023.24.008
- VernacularTitle:骨髓增生异常综合征患者骨髓γδ T细胞及其亚群的水平及意义
- Author:
Ruiting XI
1
,
2
;
Suxia GENG
;
Xin HUANG
;
Minming LI
;
Chengxin DENG
;
Yulian WANG
;
Lisi HUANG
;
Jianyu WENG
;
Xin DU
Author Information
1. 华南理工大学医学院(广州 510006)
2. 南方医科大学附属广东省人民医院(广东省医学科学院)血液科(广州 510080)
- Keywords:
myelodysplastic syndrome;
γδ T cells;
follicular helper γδ T cells;
regulatory γδ T cells;
programmed death 1;
T cell immunoglobulin and mucin-containing molecule 3
- From:
The Journal of Practical Medicine
2023;39(24):3195-3199
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the levels of γδ T cells and their subpopulations in bone marrow(BM)of patients with myelodysplastic syndrome(MDS),it aims to explore the immune deficiency status of BM microenvi-ronment in MDS patients.Methods BM samples were collected from MDS patients before and after treatment,as well as from normal donors.Multicolor flow cytometry was utilized to detect bone marrow γδ T cells and subpopulation levels.The changes of the T cell subsets after treatment were also analyzed.Results The levels of BM γδ T cells and follicular helper γδ T cells from MDS patients were significantly lower than those of normal donors(P<0.05).Among γδ T cells at different stages of differentiation,only the frequencies of na?ve γδ T cells from MDS patients decreased significantly(P = 0.037),and there was no significant difference observed about central memory,effector memory,and terminally differentiated γδ T cells in MDS patients compared to normal donors(P>0.05).Although there was a slight decrease in PD1+γδ T cells and an increase in TIM3+γδ T cells,these differences were not statistically significant(P>0.05).In patients who achieved a curative effect,the proportions of γδ T cells and naive γδ T cells increased significantly after treatment,and the effector memory γδ T cells decreased significantly after treatment(P<0.05).After treatment,85.71%(6/7)of MDS patients showed a decrease in γδ+TIM3+ T cell levels to varying degrees.Conclusions The levels of γδ T cells and their subpopulations in the BM microenvironment of patients with MDS exhibit varying degrees of abnormalities.However,in patients who receive effective treatment,these abnormal γδ T cells can recover.By detecting the levels of γδ T cells and subpopulations,we can gain insights into the immune deficiency status of MDS.This information might serve as an indicator to assess treatment efficacy and provide valuable insights for anti-tumor immunotherapy.
