Impacts of tanshinone ⅡA on lipopolysaccharide induced proliferation and apoptosis of dental pulp stem cells by regulating the Fas/FasL signaling pathway
10.3969/j.issn.1006-5725.2023.24.006
- VernacularTitle:丹参酮ⅡA调节Fas/FasL信号通路对脂多糖诱导的牙髓干细胞增殖和凋亡的影响
- Author:
Wanchao WU
1
;
Yuhuan HAN
;
Lijie LI
Author Information
1. 内蒙古医科大学附属医院口腔科(呼和浩特 010050)
- Keywords:
tanshinone ⅡA;
fatty acid synthase/fatty acid synthase ligand signaling pathway;
dental pulp stem cells;
proliferation;
apoptosis
- From:
The Journal of Practical Medicine
2023;39(24):3182-3187,3194
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the impacts of tanshinone ⅡA(Tan ⅡA)on lipopolysaccharide(LPS)induced proliferation and apoptosis of dental pulp stem cells by regulating the fatty acid synthase(Fas)/fatty acid synthase ligand(FasL)signaling pathway.Methods Identification of human pulp stem cells(hDPSCs)isolated from the third molar of 18～20 years old patients requiring orthodontics.Lps-induced hDPSCs were treated with low,medium and high doses of Tan ⅡA,and then human recombinant FasL protein(rh FasL)was used to intervene the LPS-induced hDPSCs after high dose Tan ⅡA.Proliferation and apoptosis of hDPSCs,levels of tumor necrosis factor-α(TNF-α)and interleukin(IL)-6 in hDPSCs supernatant,proliferating cell nuclear antigen(PCNA),Cleaved aspartate-specific cysteine proteinase-3(Cleaved Caspase-3),Fas,FasL protein expression were detected.Results hDPSCs were successfully isolated.In a dose-dependent manner,Tan ⅡA promoted LPS-induced proliferation,inhibited apoptosis,up-regulated PCNA protein expression,and inhibited TNF-α,IL-6 level,Cleaved Caspase-3,Fas,and FasL protein expression.The effect of rh FasL on LPS-induced dental pulp stem cells was opposite to the above indexes(P<0.05).rh FasL attenuates the effect of high-dose Tan ⅡA on pro-liferation and apoptosis of LPS-induced dental pulp stem cells.Conclusion Tan ⅡA may promote LPS induced hDPSCs proliferation and inhibit apoptosis by inhibiting the Fas/FasL signaling pathway.
