Impact of LncRNA DSCAM-AS1 on the malignant biological behaviors of thyroid cancer cells by regulat-ing the miR-150-5p/BRAF axis
10.3969/j.issn.1006-5725.2023.23.004
- VernacularTitle:LncRNA DSCAM-AS1调节miR-150-5p/BRAF轴对甲状腺癌细胞恶性生物学行为的影响
- Author:
Yun PENG
1
;
Meiling WEN
;
Yunxia LV
;
Wanzhi CHEN
;
Chun HE
;
Jian-Ping YU
;
Zhenluo DING
Author Information
1. 赣州市人民医院甲状腺外科(江西赣州 341000)
- Keywords:
lncRNA DSCAM-AS1;
miR-150-5p/BRAF axis;
thyroid cancer;
proliferation;
migra-tion;
invasion
- From:
The Journal of Practical Medicine
2023;39(23):3043-3050
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the impact of lncRNA DSCAM-AS1 on the malignant biological behaviors of thyroid cancer(TC)cells by regulating the miR-150-5p/BRAF axis.Methods The expression of DSCAM-AS1 in TC cells was detected by qRT-PCR,and the best intervention cell line was screened.;the relationship between DSCAM-AS1,BRAF and miR-150-5p targeting regulation was verified by FISH,pull down and double Luciferase reporter gene experiment;The proliferation,migration and invasion of SW579 cells were detected;Western blot was applied to detect the expression of BRAF,E-Cadherin,and vimentin proteins;the tumor formation experiment in mice was applied to verify the effect of DSCAM-AS1 on TC tumor growth.Results DSCAM-AS1 was highly expressed in TC tissue and cells(P<0.05);There is a targeted regulation relationship between DSCAM-AS1,BRAF and miR-150-5p;inhibition of DSCAM-AS1 expression or overexpression of miR-150-5p obviously inhibited the proliferation,migration,invasion,and EMT of SW579 cells(P<0.05);Inhibition of miR-150-5p expression or overexpression of BRAF reversed the inhibitory effect of inhibition of DSCAM-AS1 expression or overexpression of miR-150-5p on the malignant behavior of SW579 cells(P<0.05);in vivo experiments showed that inhibiting the expression of DSCAM-AS1 obviously inhibited the growth of transplanted tumors in mice(P<0.05).Conclusion DSCAM-AS1 is up-regulated in TC cells,inhibiting the expression of LncRNA DSCAM-AS1 can inhibit the malignant progression of TC by regulating the miR-150-5p/BRAF signaling axis.
