Association of genetic variants of m6A binding protein with the risk of gastric cancer
10.3969/j.issn.1006-5725.2023.21.024
- VernacularTitle:N6-腺苷酸甲基化结合蛋白的遗传变异与胃癌风险关联分析
- Author:
Xinyuan LU
1
;
Yanlu FENG
;
Jie LI
;
Siyi XU
;
Chengyun LI
;
Tong LIU
;
Xinhua WANG
;
Geyu LIANG
Author Information
1. 东南大学公共卫生学院,环境医学工程教育部重点实验室(南京 210009)
- Keywords:
gastric cancer;
m6A;
YTHDF1;
HNRNPA2B1;
single nucleotide polymorphism
- From:
The Journal of Practical Medicine
2023;39(21):2834-2842
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the association between single nucleotide polymorphisms(SNPs)of YTHDF1 rs6011668,HNRNPA2B1 rs2070601 and rs76558212 with the risk of gastric cancer.Methods A total of 457 cases with gastric cancer and 525 healthy controls were collected.The candidate SNPs were genotyped using Hi-SNP genotyping methods by multiplex rounds of PCR and high-throughput sequencing;the association between the three SNPs with the risk of gastric cancer was analyzed by test and Logistic regression.Multifactorial logistic regression and Risk Score(RS)model was used to analyze the influence of environmental and genetic factors on the risk of gastric cancer.Results YTHDF1rs6011668 TT genotype carriers had 3.075 times higher risk of gastric cancer than CC genotype carriers(95%CI:1.128～8.382,P = 0.028),and 2.961 times higher risk than CC/TC genotypes carriers(95%CI:1.091～8.033,P = 0.033).Subgroups-analysis revealed that TT genotype mainly increased the risk of gastric cancer in non-tea drinkers,pickled food eaters and fried food eaters(P<0.05).In addition,TT genotype carriers had the increased risk of gastric cancer infiltration,lymph node metastasis,distal metastasis and intermediate to advanced stages(P<0.05).The RS of the case and control groups were calculated by combining environmental and genetic factors.The higher the RS score,the higher the risk of gastric cancer was found in the RS quartile groups.Compared with the RS
