Inhibitory effects of allicin effective component DATS on Enterococcus faecalis bofilm:An in vitro Study
10.3969/j.issn.1001-3733.2024.01.018
- VernacularTitle:大蒜素有效成分DATS对粪肠球菌抑制作用的体外研究
- Author:
Liyuan WANG
1
;
Simin SHEN
;
Xijing BAI
Author Information
1. 300041,天津市口腔功能重建重点实验室,天津市口腔医院·南开大学口腔医院
- Keywords:
Allicin;
Dially trisulfid;
Enterococcus faecalis;
Calcium Hydroxide;
Root canal disinfection
- From:
Journal of Practical Stomatology
2024;40(1):104-108
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the antibacterial efficacy of allicin effective component,dially trisulfide(DATS),on the biofilms of Enterococcus faecalis(E.faecalis)in vitro.Methods:60 healthy single root canal permanent teeth were selected.After cleaning and autoclaving,15 teeth without infection were used in the negative control group,45 teeth were contaminated by E.faecalis and incubated for 28 days as the infection models and devided into 3 groups(n=15)as below:DATS group,Calcium hydroxide group and positive(without antibacteria treatment)control group.The minial inhibition concentration(MIC)and minial bactericidal concentrition(MBC)of DATS on E.feacalis were studied with doubling diluting method.At the day 1,2 and 7 of the exprement,the 4 mg of root canal in-ner dentin powder,5 specimens of each group were sespectively transfered into sterile bottle containing 2 mL BHI broth and cultured at 37 ℃ 5％CO2 for 72 h,the turbitity of the upper layer of liquid of the culture was measured.Results:MIC and MBC(μg/mL)of DATS to E.feacalis was 2 560 and 5 120 repsectively.At the first day,turbidity in the DATS group was the lowest(P＜0.05).At the third day,turbidity showed no difference between DATS group and negative control group(P=0.454).At the 7th day,between calcium hydroxide and the negative control group,P＜0.05.Conclusion:DATS may suppress E.faecalis in the biofilm in vitro.
