Long non-coding RNA RMST inhibits cell proliferation,migration and invasion by targeting microRNA-24-3p in OSCCs
10.3969/j.issn.1001-3733.2024.01.008
- VernacularTitle:长链非编码RNA RMST通过靶向微小RNA-24-3p抑制口腔鳞癌细胞增殖、迁移和侵袭
- Author:
Yishu LI
1
;
Shuzhen TU
Author Information
1. 215200,苏州市第九人民医院口腔科
- Keywords:
RMST;
Oral squamous cell carcinoma;
miR-24-3p;
Migration;
Invasion
- From:
Journal of Practical Stomatology
2024;40(1):47-52
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the role of long non-coding RNA rhabdomyosarcoma 2 associated transcript(lncRNA RMST)and microRNA-24-3p(miR-24-3p)in proliferation,migration and invasion of oral squamous cell carcinoma cells(OSCCs).Methods:The RMST expression in head and neck squamous cell carcinoma(HNSC)was analyzed by UALCAN data base.qRT-PCR was used to detect the expression of RMST and miR-24-3p in 5 human OSCC cell lines.CAL27 cells were in vitro cultured and allocated into con-trol group,pcDNA3.1 group(vector),pcDNA3.1-RMST+mimics-NC group(overexpression of RMST+negative control of miR-24-3p)and pcDNA3.1-RMST+miR-24-3p mimics group(overexpression of RMST+miR-24-3p).Dual luciferase reporter assay was per-formed to analyze the interaction between RMST and miR-24-3p.MTT assay,wound-healing and transwell assay were applied to de-tect the proliferation,migration and invasion of the cells.Western blot and qRT-PCR were conducted to measure the expression of E-cadherin,N-cadherin and c-myc in the cells.Results:RMST level were down-regulated in HNSC tissues and OSCC cell lines(P＜0.01).While miR-24-3p level was elevated in OSCC cell lines(P＜0.01).miR-24-3p was identified and confirmed as a potential binding partner to RMST.miR-24-3p level was lower in pcDNA3.1-RMST group than in pcDNA3.1 group(P＜0.01).The prolifera-tion,migration and invasion abilities of CAL27 cell in pcDNA3.1-RMST+mimics-NC group were lower than in pcDNA3.1 group(P＜0.01).While those of CAL27 cells in pcDNA3.1-RMST+miR-24-3p mimics group were higher than in pcDNA3.1-RMST+mimics-NC group(P＜0.01).Compared with pcDNA3.1 group,pcDNA3.1-RMST+miR-24-3p mimics showed higher expression of E-cad-herin,lower expression of N-cadherin and c-myc(P＜0.01).Compared with pcDNA3.1-RMST+miR-24-3p mimics group,pcD-NA3.1-RMST+miR-24-3p mimics group showed lower expression of E-cadherin,higher expression of N-cadherin and c-myc(P＜0.01).Conclusion:Down-expressed RMST may suppress the proliferation,migration and invasion progression of OSCC cells through targeting miR-24-3p.
