Transcriptomic analysis of chondroprogenitors in response to flow fluid shear stress
10.3969/j.issn.1001-3733.2024.01.006
- VernacularTitle:流体剪切下软骨祖细胞的转录组响应分析
- Author:
Lingfeng XU
1
;
Yuejiao ZHANG
;
Jianchang ZHANG
;
Jia YU
;
Wanqiu HUO
;
Jiali XU
;
Meiqing WANG
Author Information
1. 710032 西安,口颌系统重建与再生全国重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔疾病国际联合研究中心,空军军医大学第三附属医院口腔解剖生理学教研室
- Keywords:
Transcriptomics;
Stem cells;
Temporomandibular joint;
Osteoarthritis;
Mechanical stress
- From:
Journal of Practical Stomatology
2024;40(1):36-42
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the response of mandibular condylar chondroprogenitors to flow fluid shear stress(FFSS).Methods:Chondroprogenitors were in vitro cultured and stimulated with FFSS that can cause cell degeneration,and treated with sec-ond-generation high-throughput RNA sequencing.Differential gene expression was screened using DESeq2 software for gene ontology(GO)functional enrichment analysis,kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis and protein-protein interaction(PPI)network analysis.qRT-PCR was performed to validate the core genes screened by PPI.Results:A total of 1996 differentially expressed genes were obtained,mainly including inflammatory response and cell cycle related molecules.Among them,Actal,Atf3,Ccl2,116,Nfkbia,Ret and Vcaml were identified as the core genes.Conclusion:FFSS stimulation affects chondroprogenitor function by acting on inflammatory responses and cell cycle-related signaling pathways in chondroprogenitors.
