Regulation of GS and GLS expression by c-myc in oral epidermoid carcinoma cells and in tumor growth in nude mice
10.3969/j.issn.1001-3733.2024.01.004
- VernacularTitle:在口腔表皮样癌细胞中c-myc对GS和GLS表达的调控以及对癌瘤生长的裸鼠体内实验研究
- Author:
Qianqian ZHANG
1
;
Sihao LIU
;
Yali GUO
;
Tao WANG
Author Information
1. 710031 西安,陕西省人民医院口腔科
- Keywords:
Oral epidermoid carcinoma;
c-myc;
Glutaminase;
Glutamine synthase
- From:
Journal of Practical Stomatology
2024;40(1):26-30
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the correlation between c-myc and glutaminase(GLS)and glutamine synthase(GS)in oral epider-moid carcinoma cells in animal models.Methods:Immunohistochemistry was used to detect the expression of c-myc,GLS and GS in clinical samples of oral cancer.KB cell model with stable and high expression of c-myc was established and verified,then the KB cells were transplanted into nude mice to establish mouse tumorigenic models.The cells and nude mouse models were respectively diveded into 3 groups(n=6):normal cotnrol,empty vector and c-myc overexpression groups.The tumor growth was observed.The expression of c-myc,GLS and GS in the cells and the tumor samples was detected by immunohistochemistry.Results:c-myc,GLS and GS were highly expressed in clinical samples of oral cancer.In the cells of c-myc overexpression group c-myc mRNA expression level was sig-nificantly higher than that of empty vector control group.The tumorigenic models were formed in all nude mice of the groups,and the volume and weight of the c-myc overexpression group increased more significantly(P＜0.01),in the c-myc overexpression group c-myc was overexpressed,the expression of GLS and GS was significantly higher than that in the other 2 groups.Conclusion:c-myc is highly expressed in oral cancer,and may up-regulate GLS and GS expression.
