The Expression of TLR3 and Cytokines Induced by Poly I:C in Human Retinal Pigment Epithelial Cells.
10.4167/jbv.2007.37.4.241
- Author:
Sun Ju CHOI
1
;
Jongwook KIM
;
Su Jung PARK
;
Hyun Sook PARK
;
Kyoung Ho LEE
;
Soo Ki KIM
;
Joo Young PARK
Author Information
1. Department of Microbiology, Institute of Basic Medical Science, Yonsei University, Wonju College of Medicine, Il San Dong 162, Republic of Korea. joopark@yonsei.ac.kr
- Publication Type:Original Article
- Keywords:
Toll-like receptor3;
Poly I:C;
Viral infection;
Retinal pigment epithelium;
Interferon
- MeSH:
2',5'-Oligoadenylate Synthetase;
Cytokines*;
Enzyme-Linked Immunosorbent Assay;
Epithelial Cells*;
Humans*;
Interferons;
Interleukin-6;
Interleukin-8;
Poly I-C;
Retinal Pigment Epithelium;
Retinaldehyde*;
RNA, Messenger;
Tumor Necrosis Factor-alpha
- From:Journal of Bacteriology and Virology
2007;37(4):241-248
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
In this study, we examined the expression of Toll-like receptor3 (TLR3) by human retinal pigment epithelial cells (RPE) and determined whether exposure to the TLR3 agonist polyinosinic-polycytidylic acid (poly I:C) would induced the expression of cytokines in these cells. RT-PCR revealed that TLR3 was constitutively expressed in human RPE, and its expression was increased by treatment with poly I:C. After treatment with poly I:C, we determined the expression levels of pro-inflammatory cytokines in human RPE using RT-PCR and ELISA. We demonstrated that poly I:C treatment increased the production of TNF-alpha, IL-6, and IL-8 in human RPE. Upon exposure to poly I:C, human RPE initiated antiviral response resulting in the induction of IFN-beta mRNA expression and 2',5'-oligoadenylate synthetase mRNA expression. These results suggest that human RPE may participate in ocular defense mechanism against viral infection through TLR3.
