Role for CD40 and CD40L Expression in Generating CD8 T Cell Response to Minor Histcompatibility Antigen, H60.
- Author:
Kyoung Min JUNG
1
;
Eun Young CHOI
Author Information
- Publication Type:Original Article
- Keywords: CD8 T cell response; CD4 help; CD40; CD40L; minor histocompatibility antigen
- MeSH: Adoptive Transfer; Animals; CD40 Ligand*; Histocompatibility Antigens; Humans; Male; Memory; Mice; T-Lymphocytes
- From:Immune Network 2007;7(4):173-178
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: We studied the role for expression of CD40 and CD40L by CD4 and CD8 T cells in the generation of CD8 T cell response to minor histocompatibility antigen, H60. H60 is a cellular antigen to which CD8 responses require CD4 T cell help. METHODS: CD40- or CD40L-deficient mice were adoptively transferred with normal CD4 or CD8 T cells or with memory CD4 or CD8 T cells, and were immunized with male H60 congenic splenocytes to induce CD8 T cell response to H60. Peripheral blood CD8 T cell from the immunized mice were stained with the H60 tetramer. RESULTS: CD8 T cell response to H60 was not induced in both CD40- and CD40L-deficient mice. Adoptive transfer of CD40(+/+) CD8 T cells into CD40-deficient mice did not compensate the defect in inducing CD8 T cell response to H60, while the H60-specific CD8 T cells were activated in the CD40-deficient mice that were adoptively transferred with CD40(+/+) CD4 T cells. Adoptive transfer of CD40L(+/+) CD4 T cells into CD40L-deficient mice induced primary CD8 T cell response for H60 and the presence of CD40L(+/+) CD4 T cells was required even for memory CD8 T cells response to H60. CONCLUSION: Our results suggest that the CD40-CD40L interaction mediates the delivery of CD4 T cell help to na?ve and memory H60-specific CD8 T cells. While the expression of CD40L by CD4 T cells is essential, signaling through CD40 on CD8 T cells is not required for the induction of CD8 T cell response to H60.
