Correlation of the emm genotyping and virulence genes with the isolation sites of Group A Streptococcus strains from children with impetigo
10.3760/cma.j.cn101070-20230307-00192
- VernacularTitle:脓疱病儿童A族链球菌 emm分型、毒力基因及菌株分离部位相关性研究
- Author:
Dingle YU
1
;
Yunmei LIANG
;
Qinghua LU
;
Lili JI
;
Hesheng CHANG
;
Xia ZHANG
;
Xiaorong LIU
;
Lin MA
;
Yuejie ZHENG
;
Yonghong YANG
Author Information
1. 深圳市儿童医院呼吸科，深圳 518038
- Keywords:
Impetigo;
Group A Streptococcus;
emm genotyping;
Virulence gene;
Superantigen
- From:
Chinese Journal of Applied Clinical Pediatrics
2024;39(1):59-64
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the correlation of the emm genotypes and virulence genes with the isolation sites of Group A Streptococcus (GAS). Methods:It was a retrospective study.The specimens were collected from children with impetigo in Beijing Children′s Hospital, Capital Medical University from 2006 to 2008 for GAS isolation and identification.A total of 24 GAS strains were isolated from 16 children with impetigo, among which 7 pairs of strains were isolated from the throat and skin of 7 children, and 1 pair of strains was isolated from the vulva and skin of one child, and the remaining 8 GAS strains were isolated from the skin pus samples of 8 children.Polymerase chain reaction was applied to detect the emm genotypes and 13 virulence genes ( speA, speB, speC, speF, speG, speH, speI, speJ, speK, speL, speM, smeZ and ssa). The correlation of the emm genotypes and virulence genes with the isolation sites of GAS strains was analyzed. Results:In this study, four emm genotypes were detected, including emm1.0 (15/24), emm12.0 (4/24), emm22.0 (2/24) and emm160.0 (1/24), and one subtype emm12.19 (2/24) was detected as well.The carrying rates of 13 virulence genes speA, speB, speC, speF, speG, speH, speI, speJ, speK, speL, speM, smeZ and ssa were 58.3%, 100%, 91.7%, 100%, 50.0%, 12.5%, 54.2%, 66.7%, 16.7%, 25.0%, 12.5%, 100% and 91.7%, respectively.All strains carried 5 to 11 virulence genes and they all carried speB, speF and smeZ.There were significant differences in the carrying rate of speA and speJ among the strains with different emm genotypes (all P<0.05). There was no significant difference in the distribution of virulence genes between skin isolates and pharyngeal isolates, including the 5 pairs of strains carrying the emm1.0 genotype (all P>0.05). Conclusions:The distribution of virulence gene of GAS in children with impetigo is significantly correlated with the emm genotype, rather than the isolation site.
